Single-dose local administration of parathyroid hormone (1–34, PTH) with β-tricalcium phosphate/collagen (β-TCP/COL) enhances bone defect healing in ovariectomized rats
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Parathyroid hormone (1–34, PTH) combined β-tricalcium phosphate (β-TCP) achieves stable bone regeneration without cell transplantation in previous studies. Recently, with the development of tissue engineering slow release technology, PTH used locally to promote bone defect healing become possible. This study by virtue of collagen with a combination of drugs and has a slow release properties, and investigated bone regeneration by β-TCP/collagen (β-TCP/COL) with the single local administration of PTH. After the creation of a rodent critical-sized femoral metaphyseal bone defect, β-TCP/COL was prepared by mixing sieved granules of β-TCP and atelocollagen for medical use, then β-TCP/COL with dripped PTH solution (1.0 µg) was implanted into the defect of OVX rats until death at 4 and 8 weeks. The defected area in distal femurs of rats was harvested for evaluation by histology, micro-CT, and biomechanics. The results of our study show that single-dose local administration of PTH combined local usage of β-TCP/COL can increase the healing of defects in OVX rats. Furthermore, treatments with single-dose local administration of PTH and β-TCP/COL showed a stronger effect on accelerating the local bone formation than β-TCP/COL used alone. The results from our study demonstrate that combination of single-dose local administration of PTH and β-TCP/COL had an additive effect on local bone formation in osteoporosis rats.
KeywordsOsteoporotic bone β-Tricalcium phosphate/collagen Parathyroid hormone Defect healing Micro-CT
This study was supported by a grant from the natural science foundation for education department of Anhui Province (Grant no. KJ2017A266).
Compliance with ethical standards
Conflict of interest
All authors have no conflict of interest.
- 1.Ström O, Borgström F, Kanis John A, Compston Juliet, Cooper Cyrus, McCloskey Eugene V, Jönsson B (2011) Osteoporosis: burden, health care provision and opportunities in the EU: a report prepared in collaboration with the International Osteoporosis Foundation (IOF) and the European Federation of Pharmaceutical Industry Associations (EFPIA). Arch Osteoporos 6:59–155CrossRefGoogle Scholar
- 8.Tao ZS, Zhou WS, Tu KK, Huang ZL, Zhou Q, Sun T, Lv YX, Cui W, Yang L (2015) The effects of combined human parathyroid hormone (1–34) and simvastatin treatment on osseous integration of hydroxyapatite-coated titanium implants in the femur of ovariectomized rats. Injury 46:2164–2169. https://doi.org/10.1016/j.injury.2015.08.034 CrossRefGoogle Scholar
- 10.Tao ZS, Zhou WS, Tu KK, Huang ZL, Zhou Q, Sun T, Lv YX, Cui W, Yang L (2015) Effect exerted by teriparatide upon repair function of beta-tricalcium phosphate to ovariectomised rat’s femoral metaphysis defect caused by osteoporosis. Injury 46:2134–2141. https://doi.org/10.1016/j.injury.2015.07.042 CrossRefGoogle Scholar
- 12.Tao ZS, Tu KK, Huang ZL, Zhou Q, Sun T, Xu HM, Zhou YL, Lv YX, Cui W, Yang L (2015) Combined treatment with parathyroid hormone (1–34) and beta-tricalcium phosphate had an additive effect on local bone formation in a rat defect model. Med Biol Eng Comput. https://doi.org/10.1007/s11517-015-1402-8 Google Scholar
- 13.Tao ZS, Zhou WS, Tu KK, Huang ZL, Zhou Q, Sun T, Lv YX, Cui W, Yang L (2015) Treatment study of distal femur for parathyroid hormone (1–34) and beta-tricalcium phosphate on bone formation in critical-sized defects in osteopenic rats. J Craniomaxillofac Surg Off Publ Eur Assoc Craniomaxillofac Surg 43:2136–2143. https://doi.org/10.1016/j.jcms.2015.09.004 Google Scholar
- 14.Tao ZS, Qiang Z, Tu KK, Huang ZL, Xu HM, Sun T, Lv YX, Cui W, Yang L (2015) Treatment study of distal femur for parathyroid hormone (1–34) and beta-tricalcium phosphate on bone formation in critical size defects in rats. J Biomater Appl 30:484–491. https://doi.org/10.1177/0885328215592854 CrossRefGoogle Scholar
- 16.Mohseni M, Jahandideh A, Abedi G, Akbarzadeh A, Hesaraki S (2017) Assessment of tricalcium phosphate/collagen (TCP/collagene)nanocomposite scaffold compared with hydroxyapatite (HA) on healing of segmental femur bone defect in rabbits. Artif Cells Nanomed Biotechnol. https://doi.org/10.1080/21691401.2017.1324463
- 18.Tao ZS, Zhou WS, He XW, Liu W, Bai BL, Zhou Q, Huang ZL, Tu KK, Li H, Sun T, Lv YX, Cui W, Yang L (2016) A comparative study of zinc, magnesium, strontium-incorporated hydroxyapatite-coated titanium implants for osseointegration of osteopenic rats. Mater Sci Eng C Mater Biol Appl 62:226–232. https://doi.org/10.1016/j.msec.2016.01.034 CrossRefGoogle Scholar
- 22.Bone HG, Greenspan SL, Mckeever C, Bell N, Davidson M, Downs RW, Emkey R, Meunier PJ, Miller SS, Mulloy AL (2000) Alendronate and estrogen effects in postmenopausal women with low bone mineral density. Alendronate/Estrogen Study Group. J Clin Endocr Metab 85:720–726Google Scholar
- 26.Li Q, Wang T, Zhang G, Yu X, Zhang J, Zhou G, Tang Z (2016) A comparative evaluation of the mechanical properties of two calcium phosphate/collagen composite materials and their osteogenic effects on adipose-derived stem cells. Stem Cells Int 1–12. https://doi.org/10.1155/2016/6409546