Journal of Bone and Mineral Metabolism

, Volume 36, Issue 6, pp 668–678 | Cite as

Study of the osteogenesis effect of icariside II and icaritin on canine bone marrow mesenchymal stem cells

  • Guangming LuoEmail author
  • Biao Xu
  • Weihong Wang
  • Yong Wu
  • Ming Li
Original Article


This study aimed to identify the osteogenesis effect of icariside II (ICSII) and icaritin (ICT) in vitro. Bone marrow mesenchymal stem cells (BMSCs) were treated with ICSII and ICT in order to detect the proliferation and differentiation of BMSCs, the expression of the osteogenesis-related proteins with or without osteogenic medium (OM) and genes, Runt-related transcription factor 2 (Runx-2), osteocalcin (OCN), osteopontin (OPN), osterix, and basic fibroblast growth factor (bFGF), and the phosphorylation levels of mitogen-activated protein kinase (MAPK). We found that the optical density increased and alkaline phosphatase decreased after the BMSCs were treated with different concentrations of ICSII; however, ICT showed an opposing effect. The formation of calcium nodules was observed after the BMSCs were treated with ICSII and ICT. The expression level of osteogenesis-related proteins was enhanced following treatment with both ICSII or ICT, while the expression level of the osteogenesis-related genes Runx-2, OCN, OPN, osterix, and bFGF significantly increased with ICSII treatment (P < 0.05), and only Runx-2 and bFGF significantly increased (P < 0.01) with ICT. The expression of osteogenic differentiation-related proteins (except OPN) following treatment with ICSII + OM or ICT + OM was not notably increased. Both ICSII and ICT elevated the phosphorylation levels of MAPK/ERK, which was attenuated by GDC-0994 (an inhibitor of MAPK/ERK). Collectively, these data indicate that ICSII and ICT facilitate orientation osteogenic differentiation of BMSCs, which is most likely via the MAPK/ERK pathway. OM did not synergistically enhance the osteogenesis effect of ICSII and ICT.


Icariside II Icaritin Bone marrow mesenchymal stem cells 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.


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Copyright information

© The Japanese Society for Bone and Mineral Research and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Guangming Luo
    • 1
    Email author
  • Biao Xu
    • 1
  • Weihong Wang
    • 1
  • Yong Wu
    • 1
  • Ming Li
    • 1
  1. 1.Department of Oral and Maxillofacial SurgeryAffiliated Stomatology Hospital of Kunming Medical UniversityKunmingPeople’s Republic of China

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