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Protoplasma

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Aminooxyacetic acid (АОА), inhibitor of 1-aminocyclopropane-1-carboxilic acid (AСС) synthesis, suppresses self-incompatibility-induced programmed cell death in self-incompatible Petunia hybrida L. pollen tubes

  • L. V. KovalevaEmail author
  • E. V. Zakharova
  • G. V. Timofeeva
  • I. M. Andreev
  • Ya.Yu. Golivanov
  • L. R. Bogoutdinova
  • E. N. Baranova
  • M. R. Khaliluev
Original Article

Abstract

Self-incompatibility (SI) is genetically determined reproductive barrier preventing inbreeding and thereby providing the maintenance of plant species diversity. At present, active studies of molecular bases of SI mechanisms are underway. S-RNAse-based SI in Petunia hybrida L. is a self-/non-self recognition system that allows the pistil to reject self pollen and to accept non-self pollen for outcrossing. In the present work, using fluorescent methods including the TUNEL method allowed us to reveal the presence of markers of programmed cell death (PCD), such as DNA fragmentation, in growing in vivo petunia pollen tubes during the passage of the SI reaction. The results of statistical analysis reliably proved that PCD is the factor of S-RNAse-based SI. It was found that preliminary treatment before self-pollination of stigmas of petunia self-incompatible line with aminooxyacetic acid (AOA), inhibitor of ACC synthesis, led to stimulation of pollen tubes growth when the latter did not exhibit any hallmarks of PCD. These data argue in favor of assumption that ethylene controls the passage of PCD in incompatible pollen tubes in the course of S-RNAse-based SI functioning. The involvement of the hormonal regulation in SI mechanism in P. hybrida L. is the finding observed by us for the first time.

Keywords

S-RNAse-based self-incompatibility PCD Ethylene ABA Petunia hybrida L. 

Notes

Author contributions

L. Kovaleva and E. Zakharova wrote the manuscript; E. Zakharova, G. Timofeeva, and Ya. Golivanov performed the experiments; E. Baranova and L. B Bogoutdinova conducted imaging using TEM; I. Andreev and M. Khaliluev helped in manuscript preparation.

Funding information

This work was supported by grant from the Russian Foundation for Basic Research (RFBR, no. 17-04-00153).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • L. V. Kovaleva
    • 1
    Email author
  • E. V. Zakharova
    • 2
    • 3
  • G. V. Timofeeva
    • 1
  • I. M. Andreev
    • 1
  • Ya.Yu. Golivanov
    • 3
  • L. R. Bogoutdinova
    • 2
    • 3
  • E. N. Baranova
    • 2
  • M. R. Khaliluev
    • 2
    • 3
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia
  2. 2.All-Russia Research Institute of Agricultural BiotechnologyMoscowRussia
  3. 3.Moscow Timiryazev Agricultural AcademyRussian State Agrarian UniversityMoscowRussia

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