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Protoplasma

pp 1–13 | Cite as

Technique for the identification of osmophores in flowers of herbarium material (TIOFH)

  • Marcelo P. Hernández
  • Liliana KatinasEmail author
New Methods in Cell Biology

Abstract

The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.

Keywords

Floral osmophores Protocol Herbarium material Histochemical technique 

Notes

Acknowledgments

We acknowledge Jorge Crisci and the curators of herbaria for loan of specimens.

Funding

This study was funded by the CONICET (grant PIP 0729) and the ANPCyT (grant PICT 2012-1683), Argentina.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratorio de Morfología Comparada de Espermatófitas (LAMCE), Facultad de Ciencias Agrarias y ForestalesUNLPLa PlataArgentina
  2. 2.División Plantas Vasculares, Museo de La Plata, Facultad de Ciencias Naturales y MuseoUNLPLa PlataArgentina

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