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Protoplasma

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Pollen development in male sterile mangosteen (Garcinia mangostana L.) and male fertile seashore mangosteen (Garcinia celebica L.)

  • Pornsawan Sutthinon
  • Lacey Samuels
  • Upatham MeesawatEmail author
Original Article
  • 57 Downloads

Abstract

Mangosteen (Garcinia mangostana L.) is an economically important tropical fruit, yet the reproductive biology of this dioecious plant is complex. Male trees are not known, and female trees have sterile anthers leading to apomixis. We hypothesized that pollen abortion in mangosteen is due to altered tapetum activity during microgametogenesis. Developmental events at the cellular and sub-cellular levels during pollen development in G. mangostana were therefore examined and compared with seashore mangosteen (G. celebica L.), a closely related species with fertile anthers. In G. mangostana, the microspore mother cell had disorganized cytoplasm, including lack of Golgi apparatus and its vesicles, as well as abnormal callose wall accumulation. Globular droplets, which resembled orbicules or Ubisch bodies, were abundant in the locule, including pre-Ubisch bodies found along the tapetal plasma membrane. The tapetum of G. mangostana underwent cell death earlier than the fertile G. celebica, and during the premature death, the mitochondria had dramatically altered shapes. Low accumulation of starch in collapsed microspore mother cells and tetrad cell remnants also suggested that altered cell metabolism is related to pollen abortion in mangosteen. The present results demonstrate the importance of coordinated development between the tapetum and microspores in pollen development and provide new insights into male sterility in mangosteen (G. mangostana).

Keywords

G. celebica G. mangostana Mangosteen Male sterility Pollen abortion Tapetum 

Abbreviations

CMS

Cytoplasmic male sterility

ER

Endoplasmic reticulum

LM

Light microscope

MMC

Microspore mother cell

NFDM

Nonfat dried milk

PBS

Phosphate buffer

PCD

Programmed cell death

RER

Rough endoplasmic reticulum

TBA

Tert-butyl-ethyl alcohol series

TBST

Tris-buffered saline with 0.1%Tween-20

TEM

Transmission electron microscope

Notes

Acknowledgments

The kind assistance from Sam Livingston and Miranda Meents (UBC Botany) are really appreciated.

Funding information

This research was funded by the Science Achievement Scholarship of Thailand (SAST), Graduated School of Prince of Songkla University (PSU); the Overseas Thesis Research Scholarship from Graduated School (PSU), Thailand; and the Canadian Natural Sciences and Engineering Research Council Discovery Grant to ALS.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of SciencePrince of Songkla UniversitySongkhlaThailand
  2. 2.Department of BotanyUniversity of British ColumbiaVancouverCanada

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