Insights into the maternal pathway for Cucumber green mottle mosaic virus infection of cucurbit seeds
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Cucumber green mottle mosaic virus (CGMMV), genus Tobamovirus, is a major pathogen of cucurbits that primarily affects cucumber, melon, and watermelon crops. The aim of this study was to reveal the contribution of CGMMV-infected female flowers to disease spread. Using a fluorescent in situ hybridization (FISH) technique, we show that ovaries and ovules of CGMMV-infected cucumber and melon plants showed a CGMMV-specific fluorescence signal prior to and following anthesis. The fluorescence signal was prominent but sporadic. Ripe fruits of infected melon plants showed strong signals in the funiculus, the seed stalk, which connects the developing seed to the interior ovary wall. Importantly, in seeds, a strong fluorescence signal was observed in the perisperm-endosperm (PE) envelope, which underlies the seed coat and surrounds the embryo. Interestingly, the fluorescence signal was not uniformly distributed in the PE envelope but was localized to a specific envelope layer. These results have important epidemiological implications for CGMMV management and commercial seed production, particularly regarding the improvement of seed disinfection methods that will contribute to limit the global distribution of the virus.
KeywordsCucurbit Seeds Perisperm endosperm envelope Funiculus
We would like to thank Dr. Victor Gaba for editing the manuscript.
Contribution number 578-17 from the Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel. This work was supported by the Chief Scientist, Israel Ministry of Agriculture and the Plant Production and Marketing Board for CGMMV initiative project number 132-1740 (AD & RK).
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Conflict of interest
The authors declare that there is no conflict of interest.
- Choi GS (2001) Occurrence of two tobamovirus diseases in cucurbits and control measures in Korea. Plant Pathol J 17:243–248Google Scholar
- Dombrovsky A, and Smith E 2017. Seed transmission of tobamoviruses: aspects of global disease distribution. in: Advances in seed biology. InTech. https://www.intechopen.com/books/advances-in-seed-biology/seed-transmission-of-tobamoviruses-aspects-of-global-disease-distribution. Accessed 6 Dec 2017
- Hollings M, Komuro Y, and Tochihara H (1975) “Cucumber green mottle mosaic virus, no. 154, description of plant viruses.” Common W. Mycol. Inst./Assoc. Appl. Biol., Kew, Surrey, EnglandGoogle Scholar
- King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier, San Diego, California USAGoogle Scholar
- Kubitzki K, Kallunki J, Duretto M, and Wilson PG. 2010. Rutaceae. Pages 276–356 in: flowering plants. Eudicots. SpringerGoogle Scholar
- Ling K-S, Li R, Zhang W (2014) First report of cucumber green mottle mosaic virus infecting greenhouse cucumber in Canada. Plant Dis 98:701–701Google Scholar
- Ruzin SE (1999) Plant microtechnique and microscopy. Oxford University Press, New YorkGoogle Scholar
- Wu H, Qin B, Chen H, Peng B, Cai J, Gu Q (2011) The rate of seed contamination and transmission of Cucumber green mottle mosaic virus in watermelon and melon. Sci Agric Sin 44:527–532Google Scholar