Transcript profiling of carotenoid/apocarotenoid biosynthesis genes during corm development of saffron (Crocus sativus L.)
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The dried stigmas of saffron constitute the world’s costliest spice. Saffron has many therapeutic applications due to the presence of apocarotenoids. The latter are synthesized at different stages of development, and the biosynthetic pathway involves several genes encoding different enzymes. In order to understand the differential expression of various genes of the pathway, eight distinct developmental stages (S1-early to S8-late) were identified. The corms were assorted into three groups (I, II, and III) based on corm weight. Expression profiles of 12 carotenoid/apocarotenoid genes were studied. The expression of all genes was minimum/least in groups I and II corms during bud development. Lowest expression of carotenogenic genes (CsPSY, CsPDS, CsZDS, CsCRTISO, CsLYC-β1, CsLYC-ε, CsBCH2, and CsNCED) was observed during early stages (S1–S3) of corm growth (dormant period). In group III corms, increased expression of apocarotenoid genes (CsZCO, CsCCD2, CsUGT, and CsALDH) was observed during S4 to S8 stages (reproductive period, floral differentiation). Besides, expression profiles of genes in apical and axillary buds were also examined. Of all the genes studied, apocarotenoid biosynthesis genes (CsBCH2, CsZCO, CsCCD2, CsALDH, and CsUGT) were found to be upregulated in apical bud than in the axillary bud. The results indicated that interaction of phytohormones and sugars, mother corm reserves and the influence of internal and external factors may be contributing to the growth of saffron corm/bud. The study has laid a foundation for further research on the molecular mechanisms underlying bud dormancy/growth in saffron.
KeywordsApocarotenoids Biosynthetic pathway Bud development Crocus sativus Real-time PCR
The authors are thankful to Coordinator Bioinformatics Centre, School of Biotechnology , University of Jammu. The authors would also like to acknowledge Prof. Nikhil Churungoo, NEHU India for his valuable suggestions.
This work was supported by grants received from the Department of Biotechnology (DBT), Government of India, under Saffron Network programme (BT/PR5397/PBD/16/974/2012 and BT/PR5409/PBD/16/977/2012 from 2013 to 2018).
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- Abdulhabip O, Kaan E, Tuncay D (2017) Determination of optimum corm size for saffron (crocus sativus l.) and corm yield under the harran plain conditions. ARPN J Agric Biol Sci 12(7):236–240Google Scholar
- Abdullaev FI, Riveron-Negrete L, Caballero-Ortega H, Herdandez JM, Perez-Lopez I, Pereda-Miranda R, Espinosa-Aguirre JJ (2003) Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron (Crocus sativus L.). Toxicol in Vitro 17:731–736. https://doi.org/10.1016/S0887-2333(03)00098-5 CrossRefPubMedGoogle Scholar
- Ahrazem O, Argandona J, Fiore A, Aguado C, Lujan R, Rubio-Moraga A, Marro M, Araujo-Andrade C, Loza-Alvarez P, Diretto G, Gomez-Gomez L (2018) Transcriptome analysis in tissue sectors with contrasting crocins accumulation provides novel insights into apocarotenoid biosynthesis and regulation during chromoplast biogenesis. Sci Rep 8(1):2843. https://doi.org/10.1038/s41598-018-21225-z CrossRefPubMedPubMedCentralGoogle Scholar
- Alvarez-Orti M, Gomez-Gomez L, Rubio A, Escribano J, Pardo J, Jimenez F, Fernandez JA (2003) Development and gene expression in saffron corms. Acta Hortic 650:141–153Google Scholar
- Baba SA, Mohiuddin T, Basu S, Swarnkar MK, Malik AH, Wani ZA, Abbas N, Singh AK, Ashraf N (2015) Comprehensive transcriptome analysis of Crocus sativus for discovery and expression of genes involved in apocarotenoid biosynthesis. BMC Genomics 16:698. https://doi.org/10.1186/s12864-015-1894-5 CrossRefPubMedPubMedCentralGoogle Scholar
- Beygi HR, Kianmehr MH, Arab-Hosseini A (2006) Correlation study of dimensions and mass of saffron corm. Proceedings of 2nd International Symposium on Saffron. In: ISHS-Book of Abstracts: 53Google Scholar
- Botella O, De Juan JA, Munoz MR, Moya A, Lopez H (2002) Descripcion morfologia y ciclo anual del azafran (Crocus sativus L). Cuadernos de Fitipatologia 71:18–28Google Scholar
- Bouvier F, Suire C, Mutterer J, Camara B (2003) Oxidative remodeling of chromoplast carotenoids: identification of the carotenoid dioxygenase CsCCD and CsZCD genes involved in Crocus secondary metabolite biogenesis. Plant Cell 15:47–62. https://doi.org/10.1105/tpc.006536 CrossRefPubMedPubMedCentralGoogle Scholar
- Carmona M, Zalacain A, Alonso G (2006) El color, sabor y aroma del azafran especia. Altaban Ediciones, AlbaceteGoogle Scholar
- Castillo R, Fernandez JA, Gomez-Gomez L (2005) Implications of carotenoid biosynthetic genes in apocarotenoid formation during the stigma development of Crocus sativus and its closer relatives. Plant Physiol 139:674–689. https://doi.org/10.1104/pp.105.067827 CrossRefPubMedPubMedCentralGoogle Scholar
- Chen FC, Lee WL, Huang JZ, Huang YW (2013) Carotenoid composition and biosynthetic gene expression in Oncidium flower. The 11th Asia Pacific Orchid Conference, 888 Ishikawa, Motobu, Kunigami, Okinawa Japan, pp 1-11Google Scholar
- Dhar AK, Sapru R, Rekha K (1988) Studies on saffron in Kashmir 1. Variation in natural population and its cytological behavior. Crop Improv 15(1):48–52Google Scholar
- Dierck R, De Keyser E, De Riek J, Dhooghe E, Van Huylenbroeck J, Prinsen E, Van Der Straeten D (2016) Change in auxin and cytokinin levels coincides with altered expression of branching genes during axillary bud outgrowth in Chrysanthemum. PLoS One 11:e0161732. https://doi.org/10.1371/journal.pone.016173 CrossRefPubMedPubMedCentralGoogle Scholar
- Fernandez JA (2004) Biology, biotechnology and biomedicine of saffron. Recent Res Dev Plant Sci 2:127–159Google Scholar
- Frusciante S, Diretto G, Bruno M, Ferrante P, Pietrella M, Prado-Cabrero A, Giuliano G (2014) Novel carotenoid cleavage dioxygenase catalyzes the first dedicated step in saffron crocin biosynthesis. Proc Natl Acad Sci U S A 111(33):12246–12251. https://doi.org/10.1073/pnas.1404629111 CrossRefPubMedPubMedCentralGoogle Scholar
- Gomez-Gomez L, Parra-Vega V, Rivas-Sendra A, Segui-Simarro JM, Molina RV, Pallotti C, Rubio-Moraga A, Diretto G, Prieto A, Ahrazem O (2017) Unraveling massive crocins transport and accumulation through proteome and microscopy tools during the development of saffron stigma. Int J Mol Sci 18:76. https://doi.org/10.3390/ijms18010076 CrossRefPubMedCentralGoogle Scholar
- Koul KK, Farooq S (1984) Growth and differentiation in the shoot apical meristem of saffron plant (Crocus sativus L). J Indian Bot Soc 63:153–160Google Scholar
- Le Nard M, De Hertogh AA (1993) Bulb growth and development. In: De Hertogh AA, Le Nard M (eds) The physiology of flower bulbs. Elsevier Science Pub, Amsterdam, pp 29–43Google Scholar
- Oromi MJ (1992) Biologia de Crocus sativus L. y factores agroclimaticos que incidences el elendimiento y epoca de floracion de su cultivo en La Mancha. Doctoral thesis, Universidad de Navarra, Pamplona, SpainGoogle Scholar
- Pandey D, Pandey VS, Srivastava AP (1974) A note on the effect of the size of corms on the sprouting and flowering of saffron. Progress Hortic 6(2–3):89–92Google Scholar
- Panneerselvam R, Jaleel CA, Somasundaram R, Sridharan R, Gomathinayagam M (2007) Carbohydrate metabolism in Dioscorea esculenta (Lour.) Burk. tubers and Curcuma longa L. rhizomes during two phases of dormancy. Colloids Surf B Biointerfaces 59(1):59–66. https://doi.org/10.1016/j.colsurfb.2007.04.006 CrossRefPubMedGoogle Scholar
- Poggi LM (2009) Problematicas y nuevas perspectivas tecnologicas para la produccion de azafran. Horticultura Argentina 28:39–62Google Scholar
- Poggi LM, Portella,AJ, Pontin, MA, Molina RV (2010) Corm size and incubation effects on time to flowering and threads yield and quality in saffron production in Argentina. Acta Hortic 850: 193–198. https://doi.org/10.17660/ActaHortic.2010.850.32
- Rubio A, Rambla JL, Santaella M, Gomez MD, Orzaez D, Granell A, Gomez-Gomez L (2008) Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in beta-ionone release. J Biol Chem 283(36):24816–24825. https://doi.org/10.1074/jbc.M804000200 CrossRefPubMedPubMedCentralGoogle Scholar
- Rubio-Moraga A, Ahrazem O, Perez-Clemente RM, Gomez-Cadenas A, Yoneyama K, Lopez-Raez JA, Molina RV, Gomez-Gomez LL (2014) Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting. BMC Plant Biol 14:171. https://doi.org/10.1186/1471-2229-14-171 CrossRefPubMedPubMedCentralGoogle Scholar
- Tarantilis PA, Tsoupras G, Polissiou M (1995) Determination of saffron (Crocus sativus L.) components in crude plant extracts using high performance liquid chromatography–UV–Vis photodiode-array detection mass spectrometry. J Chromatogr A 699:107–118. https://doi.org/10.1016/0021-9673(95)00044-N CrossRefPubMedGoogle Scholar
- Von Lintig J, Welsch R, Bonk M, Giuliano G, Batschauer A, Kleinig H (1997) Light-dependent regulation of carotenoid biosynthesis occurs at the level of phytoene synthase expression and is mediated by phytochrome in Sinapis alba and Arabidopsis thaliana seedlings. Plant J 12(3):625–634. https://doi.org/10.1046/j.1365-313X.1997.d01-16.x CrossRefGoogle Scholar