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Protoplasma

pp 1–15 | Cite as

Cytotoxic allelochemicals induce ultrastructural modifications in Cassia tora L. and mitotic changes in Allium cepa L.: a weed versus weed allelopathy approach

  • Waseem MushtaqEmail author
  • Quratul Ain
  • M. B. Siddiqui
  • Khalid Rehman HakeemEmail author
Original Article

Abstract

The stress induced by allelochemicals present in stem aqueous extract (SAE) of Nicotiana plumbaginifolia on alterations in growth, ultrastructure on Cassia tora L., and mitotic changes on Allium cepa L. were inspected. Application of SAE at different concentrations (0.5, 1, 2, and 4%) expressively reduced the growth of C. tora in terms of seedling length and dry biomass. Moreover, the ultrastructural variations induced in the epidermis of Cassia leaf (adaxial and abaxial surface) of 15-day-old saplings were analyzed through scanning electron microscopy (SEM). The variations noticed are rupturing and shrinking of cells along epidermis; damaged margins, extensively curled leaf apex along with the appearance of puff-like structures, grooves, and thread-like structures on the leaf surface. The epidermal cells of samples exposed to treatment no longer appear smooth relative to control, besides showing necrosis as well. Upon exposure to different concentrations of extract, A. cepa root tip cells showed aberrations in chromosome arrangement and disparity in the shape of the interphase and prophase nuclei along various phases of mitotic cycle as compared to control. The mitotic index (MI) showed a concentration-dependent decline in onion root tips exposed to SAE. The aberrations appearing frequently were formation of multinucleated cells, sticky metaphase and anaphase with bridges, sticky telophase, disturbed polarity, etc. The results also show the induction of elongated cells, giant cells, and cells with membrane damage by extract treatment. To our knowledge, this is the first gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic extract of N. plumbaginifolia stem. Overall, 62 compounds were reported, covering 99.61% of the entire constituents, which can be considered responsible for the allelopathic suppression of C. tora. The chief component was 4-tert-butylcalix[4]arene with the highest composition of 19.89%, followed by palmitic acid (12.25%), palmitoleic acid (8.23%), precocene 2 (7.53%), isophytyl acetate (4.01%), and betastigmasterol (3.95%).

Keywords

Allelochemicals GC-MS analysis Scanning electron microscopy Mitotic changes 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abouziena HF, Haggag WM (2016) Weed control in clean agriculture: a review1. Planta 34(2):377–392Google Scholar
  2. Ajaib M, Fatima S, Kamran SH, Khan KM, Perveen S, Shah S (2016) Comparative antidiabetic evaluation of different parts of Nicotiana Plumbajnifolia in alloxan induced diabetic mice. J Chem Soc Pak 38:1267–1267Google Scholar
  3. Ahmed SM, Abdelgaleil SAM (2005) Antifungal activity of extracts and sesquiterpene lactones from Magnolia grandiflora L. (Magnoliaceae). Int J Agric Biol 7:638–642Google Scholar
  4. Anonymous (1966) Nicotiana L. In: Zaheer SH, Prasad B, Chopra RN, Santapau H, Krishnan MS, Deshaprabhu SB (eds) The wealth of India, raw materials, vol VII. CSIR Publications, New Delhi, India, p 46Google Scholar
  5. Arora K (2013) Mitotic aberrations induced by Cassia ocidentalis L. in Allium cepa L. root tip cells. Indian J Fund Appl Life Sci 3:1–4Google Scholar
  6. Arora N, Pandey-Rai S (2014) GC–MS analysis of the essential oil of Celastrus paniculatus Willd. Seeds and antioxidant, anti-inflammatory study of its various solvent extracts. Ind Crop Prod 61:345–351Google Scholar
  7. Celik TA, Aslanturk OS (2010) Evaluation of cytotoxicity and genotoxicity of Inula viscosa leaf extracts with Allium test. J BioMed Biotechnol, Vol 2010, doi: https://doi.org/10.1155/2010/189252,
  8. Bajwa AA (2014) Sustainable weed management in conservation agriculture. Crop Prot 65:105–113Google Scholar
  9. Bergin D (2011) Weed control options for coastal sand dunes: a review. New Zealand Forest research institute LTD, pp. 5–13Google Scholar
  10. Blum U (2011) Plant-plant allelopathic interactions: phenolic acids, cover crops and weed emergence. Springer, LondonGoogle Scholar
  11. Borghetti F, Silva LCR, Pinheiro JD, Varella BB, Ferreira AG (2005) Aqueous leaf extract properties of Cerrado species in Central Brazil. In: Harper JDI, An M, Wu H, Kent JH (eds) Proceedings and selected papers: fourth world congress on allelopathy. The George Stutt University, Wagga, pp 388–390Google Scholar
  12. Borghetti F, Lima ECD, Silva LCR (2013) A simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties. Acta Bot Bras 27:50–53Google Scholar
  13. Devi S, Thoppil JE (2016) Cytotoxic studies and phytochemical analysis of Orthosiphon thymiflorus (Roth) sleesen. Int J Pharm Pharm Sci 8(2):249–255Google Scholar
  14. Chauvel B, Guillemin JP, Gasquez J, Gauvrit C (2012) History of chemical weeding from 1944 to 2011 in France: changes and evolution of herbicide molecules. Crop Prot 42:320–326Google Scholar
  15. Duke SO, Dayan FE, Rimando RM, Scharder KK, Aliotta G, Oliva A, Romangini JG (2002) Chemicals from nature for weed management. Weed Sci 50:138–151Google Scholar
  16. Duncan DB (1955) Multiple range and multiple F-tests. Biometrics 11:1–42Google Scholar
  17. Ercoli L, Masoni A, Pampan S, Arduini I (2007) Allelopathic effects of rye, brown mustard and hairyn vetch on redroot pigweed, common lambsquarter and knotweed. Allelopathy J 19:249–256Google Scholar
  18. Farooq M, Jabran K, Cheema ZA, Wahid A, Siddique KHM (2011) The role of allelopathy in agricultural pest management. Pest Manag Sci 67:493–506Google Scholar
  19. Fiskesjo G (1985) The Allium test as a standard in environmental monitoring. Hereditas 102:99–112Google Scholar
  20. Fiskesjo G (1997) Allium test for screening chemicals: evaluation of cytologic parameters. In: Wang W, Gorsuch JW, Hughes JS (eds) Plants for environmental studies. CRC Publishers, Boca Raton, pp 308–333Google Scholar
  21. Gaba S, Gabriel E, Chadœuf J, Bonneu F, Bretagnolle V (2016) Herbicides do not ensure for higher wheat yield, but eliminate rare plant species. Sci Reports 6:30112Google Scholar
  22. Ghareib HRA, Hamed MSA, Ibrahim OH (2010) Antioxidative effects of acetone fraction and vanillic acid from Chenopodium murale on tomato plants. Weed Biol Manag 10:64–72Google Scholar
  23. Grisi PU, Gualtieri SCJG, Pereira VCP, Imatomi SAM (2012) Phytotoxic activity of Sapindus saponaria L. leaf and stem bark on initial growth of Triticum aestivum L. Commun Plant Sci 2:2237–4027Google Scholar
  24. Guglielmini AC, Verdu AM, Satorre EH (2017) Competitive ability of five common weed species in competition with soybean. Int J Pest Manag 2;63(1):30-6Google Scholar
  25. Gulzar S, Siddiqui MB (2014) Evaluation of allelopathic effect of Eclipta alba (L.) Hassk on biochemical activity of Amaranthus spinosus L., Cassia tora L. and Cassia sophera L. Afr. J. Environ Sci Techn 8:1–5Google Scholar
  26. Gulzar A, Siddiqui MB, Bi S (2016) Phenolic acid allelochemicals induced morphological, ultrastructural, and cytological modification on Cassia sophera L. and Allium cepa L. Protoplasma 253(5):1211–1221Google Scholar
  27. Harker KN (2013) Slowing weed evolution with integrated weed management. Can J Plant Sci 93:759–764Google Scholar
  28. Havey MJ (2002) Genome organization in Allium. In: Rabinowitch HD, Currah L (eds) Allium crop science. Recent Advances. CABI Publishing, United Kingdom, pp 59–79Google Scholar
  29. Hussain MI, Gonzalez L, Reigosa MJ (2011) Allelopathic potential of Acacia melanoxylon R. Br. On the germination and root growth of native species. Weed Biol Manag 11:18–28Google Scholar
  30. Ishak MS, Sahid I (2014) Allelopathic effects of the aqueous extract of the leaf and seed of Leucaena leucocephala on three selected weed species. AIP Conference Proceedings 1614(1):659–664Google Scholar
  31. Jabran K, Mahajan G, Sardana V, Chauhan BS (2015) Allelopathy for weed control in agricultural systems. Crop Prot 72:57–65Google Scholar
  32. Javed S, Javaid A, Shoaib A (2014) Herbicidal activity of some medicinal plants extracts against Parthenium hysterophorus L. Pak J Weed Sci Res 20(3):279–291Google Scholar
  33. Khanh TD, Chung MI, Xuan TD, Tawata S (2005) The exploitation of crop allelopathy in sustainable agricultural production. J Agron Crop Sci 191(3):172–184Google Scholar
  34. Khanh TD, Elzaawely AA, Chung IM, Ahn JK, Tawata S, Xuan TD (2007) Role of allelochemicals for weed management in rice. Allelopathy J 19(1):85–96Google Scholar
  35. Khalila A,, Maslata A , Hafiza A, Mizyedb S, Ashram M (2014) Zeitschrift für Naturforschung C, 64: (3-4) 167–175Google Scholar
  36. Knapp S, Clarkson JJ (2004) (1642) Proposal to conserve the name Nicotiana plumbaginifolia against N. pusilla, N. humilis and N. tenella (Solanaceae). Taxon 53(3):844–846Google Scholar
  37. Kong CH, Li HB, Hu F, Xu XH (2006) Allelochemicals released by rice roots and residues in soil. Plant Soil 288:47–56Google Scholar
  38. Kumar DS, Chakrabarty D, Verma AK, Banerji BK (2011) Gamma ray induced chromosomal aberrations and enzyme related defense mechanism in Allium cepa L. Caryologia 64(4):388–397Google Scholar
  39. Ladhari A, Omezzine F, DellaGreca M, Zarrelli A, Haouala SZR (2013) Phytotoxic activity of Cleome arabica L. and its principal discovered active compounds. South Afr J Bot 88:341–351Google Scholar
  40. Li ZH, Wang Q, Ruan X, Pan CD, Jiang DA (2010) Phenolics and plant allelopathy. Molecules 15(12):8933–8952Google Scholar
  41. Liu Q, Lu D, Jin H, Yan Z, Li X, Yang X, Guo H, Qin B (2014) Allelochemicals in the rhizosphere soil of Euphorbia himalayensis. J Agri Food Chem 62:8555–8561Google Scholar
  42. Miyake Y. (2009) Plant growth inhibitor. Japan patent no 2009274970. Tokyo: Japan patent officeGoogle Scholar
  43. Mohamed FI, El-Ashry ZM (2012) Cytogenetic effect of allelochemicals Brassica nigra L. extracts on Pisum sativum L. World Appl Sci J 20(3):344–353Google Scholar
  44. Muscolo A, Panuccio MR, Sidari M (2001) The effect of phenols on respiratory enzymes in seed germination respiratory enzyme activities during germination of Pinus laricio seeds treated with phenols extracted from different forest soils. Plant Growth Regul 35:31–35Google Scholar
  45. Mushtaq W, Quratul-Ain, Siddiqui MB (2018) Screening of alleopathic activity of the leaves of Nicotiana plumbaginifolia Viv. on some selected crops in Aligarh, Uttar Pradesh, India. Int J Photochem Photobiol 2(1):1–4Google Scholar
  46. Nefic H, Musanovic J, Metovic A, Kurteshi K (2013) Chromosomal and nuclear alterations in root tip cells of Allium cepa L. induced by alprazolam. Med Arch 67(6):388Google Scholar
  47. Nwakanma NMC, Okoli BE (2010) Cytological effects of the root extracts of Boerhaavia diffusa on root tips of Crinum jagus. Eurasia J Biosci 4:105–111Google Scholar
  48. Oerke EC (2006) Crop losses to pests. J Agr Sci 144(1):31–43Google Scholar
  49. Ogata T., Hamachi M., Nishi K. (2008) Organic herbicide for Paddy field. Japan patent no 2008050329. Tokyo: Japan patent officeGoogle Scholar
  50. Omezzine F, Ladhari A, Haouala R (2014) Physiological and biochemical mechanisms of allelochemicals in aqueous extracts of diploid and mixoploid Trigonella foenum-graecum L. South Afr J Bot 93:167–178Google Scholar
  51. Pan L, Li X, Yan Z, Guo H, Qin B (2015) Phytotoxicity of umbelliferone and its analogs: structure-activity relationships and action mechanisms. Plant Physiol Biochem 97:272–277Google Scholar
  52. Pawlowski A, Kaltchuk-Santos E, Zini CA, Caramao CB, Soares GLS (2012) Essential oils of Schinus terebinthifolius and S. molle (Anacardiaceae): mitodepressive and aneugenic inducers in onion and lettuce root meristems. South Afr J Bot 80:96–103Google Scholar
  53. Pina GDO, Borghetti F, Silveira CES e, Pereira LAR (2009) Effects of Eugenia dysenterica leaf extracts on the growth of sesame and radish. Allelopathy J 23:313–322Google Scholar
  54. Proestos C, Sereli D, Komaitis M (2006) Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS. Food Chem 95:44–52Google Scholar
  55. Rawat LS, Maikhuri RK, Negi VS, Bahuguna YM, Pharswan DS, Maletha A (2016) Allelopathic performance of medicinal plants on traditional oilseed and pulse crop of Central Himalaya, India. Natl Acad Sci Lett 39(3):141–144Google Scholar
  56. Reigosa MJ, Souto XC, Gonz’lez L (1999) Effect of phenolic compounds on the germination of six weeds species. J Chem Ecol 28:83–88Google Scholar
  57. Rice EL (1974) Allelopathy. Academic Press, New York, p 353Google Scholar
  58. Rueda-Ayala V, Rasmussen J, Gerhards R, Fournaise NE (2011) The influence of post-emergence weed harrowing on selectivity, crop recovery and crop yield in different growth stages of winter wheat. Weed Res 51(5):478–488Google Scholar
  59. Sadia S, Qureshi R, Khalid S, Nayyar BG, Zhang JT (2015) Role of secondary metabolites of wild marigold in suppression of Johnson grass and Sun spurge. Asian Pac J Trop Biomed 5(9):733–737Google Scholar
  60. Salarti MA, Kato-Noguchi H (2010) Allelopathic potential of methanol extract of Bangladesh rice seedlings. Asian J Crop Sci 2(2):70–77Google Scholar
  61. Sarwar N, Jamil FF, Parveen R (2001) Accumulation of phytoalexins and phenylalanine ammonia lyase in chickpea after inoculation with Ascochyta rabiei and their role in defence mechanism. Pak J Bot 33:373–382Google Scholar
  62. Schulz M, Kussmann P, Knop M, Kriegs B, Gresens F, Eichert T, Ulbrich A, Marx F, Fabricius H, Goldbach H, Noga G (2007) Allelopathic monoterpenes interfere with Arabidopsis thaliana cuticular waxes and enhance transpiration. Plant Signal Behav 2:231–239Google Scholar
  63. Sharma S, Devkota A (2015) Allelopathic potential and phytochemical screening of four medicinal plants of Nepal. Sci World 12(12):56–61Google Scholar
  64. Singh HP, Batish DR, Kohli RK (2003) Allelopathic interactions and allelochemicals: new possibilities for sustainable weed management. Crit Rev Plant Sci 22(3–4):239–311Google Scholar
  65. Singh A, Singh D, Singh NB (2009) Allelochemical stress produced by aqueous leachate of Nicotiana plumbaginifolia Viv. Plant Gr Reg 58:163–171Google Scholar
  66. Singh A, Singh D, Singh NB (2015) Allelopathic activity of Nicotiana plumbaginifolia at various phenological stages on sunflower. Allelopath J 1:36(2)Google Scholar
  67. Sodaeizadeh H, Rafieiolhossaini M, Damme PV (2010) Herbicidal activity of a medicinal plant, Peganum harmala L., and decomposition dynamics of its phytotoxins in the soil. Ind Crop Prod 31(2):385–394Google Scholar
  68. Soltys D, Langwald AR, Gniazdowska A, Wiśniewska A, Bogatek (2011) Inhibition of tomato (Solanum lycopersicum L.) root growth by cyanamide is due to altered cell division, phytohormone balance and expansin gene expression. Planta 236:1629–1638Google Scholar
  69. Taek–Keun OH, Shinogi Y, Chikushi J, Yong–Hwan LE, Choi B (2012) Effect of aqueous extract of biochar on germination and seedling growth of lettuce (Lactuca sativa L.). J Fac Agr Kyushu Univ 57(1):55–60Google Scholar
  70. Teerarak M, Laosinwattana C, Charoenying P (2010) Evaluation of allelopathic, decomposition and cytogenetic activities of Jasminum officinale L. f. var. grandiflorum (L.) Kob. on bioassay plants. Bioresour Technol 101:5677–5684Google Scholar
  71. Tuyen PT, Xuan TD, Tu Anh TT, Mai Van T, Ahmad A, Elzaawely AA, Khanh TD (2018) Weed suppressing potential and isolation of potent plant growth inhibitors from Castanea crenata Sieb. et Zucc. Molecules 23(2):345Google Scholar
  72. Vyvyan JR (2002) Allelochemicals as leads for new herbicides and agrochemicals. Tetrahedron 58:1631–1646Google Scholar
  73. Young SL, Pierce FJ, Nowak P (2014) Introduction: scope of the problem—rising costs and demand for environmental safety for weed control. Automation: the future of weed control in cropping systems. Springer, pp. 1-8Google Scholar
  74. Zimdahl RL (2013) Fundamentals of weed science, 4th edn. Academic Press, San Diego, p 664Google Scholar
  75. Zeng RS (2014) Allelopathy—the solution is indirect. J Chem Ecol 40(6):515–516Google Scholar
  76. Zhang XH, Lang DY, Chen J, Zhao YS, Wu XL, Fu XY (2014) Autotoxicity of aqueous extracts from plant of cultivated Astragalus membranaceus var. mongholicus. Zhong yao cai = J Chinese Med Mat 37(2):187–191Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Alleopathy Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Biological Sciences, Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia

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