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Glandular trichomes of the leaves in three Doronicum species (Senecioneae, Asteraceae): morphology, histochemistry, and ultrastructure

  • Lyudmila E MuravnikEmail author
  • Olga V Kostina
  • Anna A Mosina
Original Article


Two types of glandular tichomes (GTs) develop on the leaves in three Doronicum species. The purpose of the work was to establish common and distinctive morphological, anatomical, histochemical, and ultrustructural features of the trichomes. It turned out that differences between types of trichomes are more significant than interspecific ones. For each Doronicum species, differences between GTs of two types include the dimensions, intensity of coloration by histochemical dyes, as well as ultrastructural features of the cells. The GTs of the first type are higher than GTs of the second type. Two to three upper cell layers of the first trichomes develop histochemical staining, whereas in the second ones, only apical cells give a positive histochemical reaction. In all trichomes, polysaccharides, polyphenols, and terpenoids are detected. In the GTs of the first type, polysaccharides are synthesized in larger quantity; in the GTs of the second type, synthesis of the secondary metabolites predominates. Main ultrastructural features of the GTs of the first type include proliferation of RER and an activity of Golgi apparatus denoting the synthesis of enzymes and pectin; however, development of SER, diversiform leucoplasts with reticular sheaths, and chloroplasts with peripheral plastid reticulum also demonstrate the synthesis of lipid substances. The ultrastructural characteristics of the second type GTs indicate the primary synthesis of lipid components. Secretion is localized in a periplasmic space of the upper cell layers. The secretory products pass through the cell wall, accumulate in the subcuticular cavity, and rupture it.


Glandular trichomes Morphology Anatomy Histoxhemistry Ultrastructure Secretion 



We appreciate the Core Centre “Cell and Molecular Technology in the Plant Science” at the Komarov Botanical Institute (St. Petersburg) for provision of equipment for light and electron microscopy. The present study was carried out within the framework of the institutional research project (01201255608) of the Komarov Botanical Institute of the Russian Academy of Sciences. We also thank the two anonymous reviewers for their comments on this manuscript.

Author contribution statement

LEM conceived and coordinated all experiments, analyzed data, and wrote the manuscript; OVK and AAM contributed to histochemistry and microscopy.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Authors and Affiliations

  1. 1.Laboratory of Plant Anatomy and MorphologyKomarov Botanical Institute of the Russian Academy of SciencesSt. PetersburgRussia

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