Protoplasma

, Volume 255, Issue 3, pp 803–817 | Cite as

The inhibition of polyamine biosynthesis weakens the drought tolerance in white clover (Trifolium repens) associated with the alteration of extensive proteins

  • Zhou Li
  • Yan Zhang
  • Dandan Peng
  • Yan Peng
  • Xinquan Zhang
  • Xiao Ma
  • Linkai Huang
  • Yanhong Yan
Original Article
First Online:
Received:
Accepted:

Abstract

Changes of endogenous polyamine (PA) levels could be a key adaptive response to drought in plants. White clover pretreated with or without dicyclohexylamine (DCHA), an inhibitor of PA biosynthesis, was subjected to drought stress induced by 18% polyethylene glycol 6000 for 8 days in controlled growth chambers. Results showed that drought stress significantly increased endogenous PA content, whereas DCHA significantly decreased PA accumulation under drought stress. The attenuate PA biosynthesis was unfavorable for plant growth and drought tolerance, as reflected by significantly lower relative water content, relative growth rate, instantaneous water use efficiency, and cell membrane stability in leaves in response to drought. On the basis of proteomic analysis, the inhibition of PA synthesis decreased the accumulation of many key differentially expressed proteins including (1) ribosomal structure and biogenesis: elongation factor, ribosomal protein S10E, and 30S ribosomal protein; (2) amino acid transport and metabolism: cysteine synthase, delta-1-pyrroline-5-carboxylate synthetase, and glutamate decarboxylase; (3) carbohydrate metabolism and energy production: photosystem apoprotein, sucrose-phosphate synthase, phosphogluconate dehydrogenase, sucrose-phosphatase, NADH oxidoreductase, and ATP synthase; (4) antioxidant metabolism: catalase, peroxidase I, ascorbate peroxidase, and glutathione S-transferase; and (5) other biological processes: heat shock protein 70, heat shock protein 90, and calcium-dependent protein kinase associated with the decreased drought tolerance in white clover. These findings indicate that PAs play a critical role in the regulation of growth, ribosome, amino acid and energy metabolism, and antioxidant reactions in white clover under drought stress. Drought-induced increases in endogenous PAs could be one of key adaptive responses against drought stress in white clover.

Keywords

Differentially expressed proteins Growth Metabolic pathway iTRAQ-based proteome Photosynthesis 

Abbreviations

APX

Ascorbate peroxidase

CAT

Catalase

CDPK

Calcium-dependent protein kinase

Chl

Chlorophyll

DEPs

Differentially expressed proteins

DCHA

Dicyclohexylamine

GST

Glutathione S-transferase

HPLC

High-performance liquid chromatograph

HSPs

Heat shock proteins

LSD

Least significant difference

MDA

Malondialdehyde

PAs

Polyamines

Pn

Net photosynthetic rate

POD

Peroxidase

Put

Putrescine

ROS

Reactive oxygen species

RWC

Relative water content

RGR

Relative growth rate

SOD

Superoxide dismutase

Spd

Spermidine

Spm

Spermine

Tr

Transpiration rate

WUE

Water use efficiency

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Notes

Authors’ contribution

Prof. Yan Peng designed the experiments and provided materials and funding; Dr. Zhou Li performed experiments and wrote the manuscript; Dr. Zhou Li, Yan Zhang, and Dandan Peng analyzed the data; Prof. Xinquan Zhang and Xiao Ma provided reagents and materials; Prof. Linkai Huang and Yanhong Yan improved the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest

Supplementary material

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ESM 1 (DOCX 16.4 kb)
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ESM 2 (DOCX 17.1 kb)
709_2017_1186_MOESM3_ESM.docx (42 kb)
ESM 3 (DOCX 42.3 kb)

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© Springer-Verlag GmbH Austria, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Grassland Science, College of Animal Science and TechnologySichuan Agricultural UniversityChengduChina

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