Electrochemical determination of indole-3-acetic acid and indole-3-butyric acid using HPLC with carbon felt detector

  • Hana DejmkovaEmail author
  • Marjorie de Araújo Daniel
Original Paper


Method for the determination of two auxins, indole-3-acetic acid and indole-3-butyric acid, is developed using HPLC with electrochemical detection. As the working electrode, recently developed carbon felt detector with high conversion degree was employed. Optimum conditions, i.e. separation on 125-4 Lichrospher 100, RP-18 column with mobile phase consisting of buffer pH 4.0 and methanol (40:60, v/v) and detection at potential + 1.5 V, allow to obtain linear concentration dependences in the concentration range from 1 to 100 µmol dm−3, with determination limits of 1.1 µmol dm−3 and 1.8 µmol dm−3 for indole-3-acetic and -butyric acid, respectively. Applicability of the method was verified by the determination of both analytes in rooting preparation; the results were in agreement with the reference method, although not with the values declared by the manufacturer.

Graphical abstract


Auxins Electrochemistry High-pressure liquid chromatography Coulometry 



This research was financially supported by The Czech Science Foundation (project GA17-03868S).


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.UNESCO Laboratory of Environmental Electrochemistry, Department of Analytical Chemistry, Faculty of ScienceCharles UniversityPrague 2Czech Republic
  2. 2.Federal University of Rio Grande do SulPorto AlegreBrazil

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