Voltammetric behavior of a candidate anticancer drug roscovitine at carbon electrodes in aqueous buffers and a cell culture medium
- 42 Downloads
Roscovitine is a candidate anticancer drug acting as an inhibitor of cyclin-dependent kinases which are involved in cell-cycle control and in apoptosis triggering. In our present study,we show for the first time that roscovitine yields a well-developed, analytically useful signal due to its irreversible electrochemical oxidation in alkaline range of pH (while in acidic media, its oxidation process is apparently rather complex and corresponding voltammetric signals are less suitable for analytical purposes). We demonstrate the possibility of voltammetric determination of the drug in buffer solutions (down to submicromolar concentrations of roscovitine) as well as in cell culture medium (down to micromolar concentrations) using in-house-fabricated pyrolytic graphite or screen-printed carbon electrodes.
KeywordsRoscovitine Anticancer drug Carbon electrodes Voltammetry Cell culture medium
This research was supported by the Czech Science Foundation (project P206/12/G151). Support from the Academy of Sciences of the Czech Republic (RVO: 61388963) and Ministry of Education, Youth and Sports of the Czech Republic under project CEITEC 2020 (LQ1601) is acknowledged. The authors thank Metrohm CZ for efficient technical, material and intellectual support.
- 10.Raynaud FI, Fischer PM, Nutley BP, Goddard PM, Lane DP, Workman P (2004) Mol Cancer Ther 3:353Google Scholar
- 21.Hasoň S, Daňhel A, Schwarzová-Pecková K, Fojta M (2018) Carbon electrodes in electrochemical analysis of biomolecules and bioactive substances: roles of surface structures and chemical groups. In: Nikolelis DP, Nikoleli G-P (eds) nanotechnology and biosensors. Elsevier Inc, Amsterdam, p 51Google Scholar
- 24.DMEM LM-D1111 high glucose medium. http://www.biosera.com/wp-content/uploads/2014/09/DMEM-High-glucose-1.pdf