Monatshefte für Chemie - Chemical Monthly

, Volume 150, Issue 3, pp 461–467 | Cite as

Voltammetric behavior of a candidate anticancer drug roscovitine at carbon electrodes in aqueous buffers and a cell culture medium

  • Petr Orság
  • Luděk Havran
  • Lukáš Fojt
  • Jan Coufal
  • Václav Brázda
  • Miroslav FojtaEmail author
Original Paper


Roscovitine is a candidate anticancer drug acting as an inhibitor of cyclin-dependent kinases which are involved in cell-cycle control and in apoptosis triggering. In our present study,we show for the first time that roscovitine yields a well-developed, analytically useful signal due to its irreversible electrochemical oxidation in alkaline range of pH (while in acidic media, its oxidation process is apparently rather complex and corresponding voltammetric signals are less suitable for analytical purposes). We demonstrate the possibility of voltammetric determination of the drug in buffer solutions (down to submicromolar concentrations of roscovitine) as well as in cell culture medium (down to micromolar concentrations) using in-house-fabricated pyrolytic graphite or screen-printed carbon electrodes.

Graphical abstract


Roscovitine Anticancer drug Carbon electrodes Voltammetry Cell culture medium 



This research was supported by the Czech Science Foundation (project P206/12/G151). Support from the Academy of Sciences of the Czech Republic (RVO: 61388963) and Ministry of Education, Youth and Sports of the Czech Republic under project CEITEC 2020 (LQ1601) is acknowledged. The authors thank Metrohm CZ for efficient technical, material and intellectual support.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Petr Orság
    • 1
  • Luděk Havran
    • 1
  • Lukáš Fojt
    • 1
  • Jan Coufal
    • 1
  • Václav Brázda
    • 1
  • Miroslav Fojta
    • 1
    • 2
    Email author
  1. 1.Institute of BiophysicsCzech Academy of SciencesBrnoCzech Republic
  2. 2.Central European Institute of TechnologyMasaryk UniversityBrnoCzech Republic

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