Monatshefte für Chemie - Chemical Monthly

, Volume 149, Issue 9, pp 1709–1718 | Cite as

Isotachophoretic determination of amino acids after their conversion to hydroxy acids

  • Michaela KovářováEmail author
  • Martin Bartoš
  • Tomáš Mikysek
  • Dana Hloušková
  • Blanka Hyhlíková
  • Jana Vladyková
Original Paper


In this article, determination of amino acids by capillary isotachophoresis based on their derivatization (van Slyke reaction) is presented. Hence, the suggested method deals with reaction of amino acids with nitrous acid forming corresponding hydroxy acids. This reaction was used in isotachophoretic analysis for the first time. Optimal conditions represented by electrolyte system for separation of hydroxy acids, 0.01 mol/dm3 HCl, 0.05% hydroxyethyl cellulose, with β-alanine (leading electrolyte of pH 3.6) and 0.01 mol/dm3 valeric acid with sodium hydroxide (terminating electrolyte of pH 7.24), were chosen; furthermore, other critical details of the analysis are also described. Developed method brings original results and shows applicability on analysis of amino acids in relatively simple mixtures, especially in food supplements.

Graphical abstract


Amines Diazo compounds Electrophoresis Zwitterions 


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Michaela Kovářová
    • 1
    Email author
  • Martin Bartoš
    • 1
  • Tomáš Mikysek
    • 1
  • Dana Hloušková
    • 1
  • Blanka Hyhlíková
    • 1
  • Jana Vladyková
    • 1
  1. 1.Institute of Analytical Chemistry, University of PardubicePardubiceCzech Republic

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