Monatshefte für Chemie - Chemical Monthly

, Volume 149, Issue 9, pp 1679–1684 | Cite as

Simultaneous determination of sinapic acid and tyrosol by flow-injection analysis with multiple-pulse amperometric detection

  • Dmytro BavolEmail author
  • Anastasios Economou
  • Jiri Zima
  • Jiri Barek
  • Hana Dejmkova
Original Paper


This work describes a simple, fast (frequency of 170 injections h−1), and low-cost method for the simultaneous determination of two antioxidants, sinapic acid and tyrosol, using multiple-pulse amperometric detection at a glassy carbon electrode incorporated in a flow-injection analysis cell. A sequence of potential pulses was selected to detect sinapic acid and tyrosol separately in the course of a single injection step. During the characterization of electrochemical detection, conditions for the determination of the two antioxidants (such as the injected volume and the flow rate) were studied and the analytical figures of merit were calculated. The repeatability (expressed as %) RSD was < 4.0% (n = 10) and excellent linearity was obtained across two concentration ranges from 1.0 to 100 μM; the limits of detection of sinapic acid and tyrosol were around 1.0 μM.

Graphical abstract


Electrochemistry Flow-injection analysis Glassy carbon electrode Oxidations Voltammetry 



Financial support of the Czech Science Foundation (Project P206/12/G151) is acknowledged and Erasmus programme.


  1. 1.
    Charrouf Z, Guillaume D (2007) Am J Food Technol 2:679CrossRefGoogle Scholar
  2. 2.
    Anthony NR, Samuel SM (2008) Research topics in agricultural and applied economics, 1st edn. Sharjah, United Arab EmiratesGoogle Scholar
  3. 3.
    Lucas R, Comelles F, Alcantara D, Maldonado OS, Curcuroze M, Parra JL, Morales JC (2010) J Agric Food Chem 58:8021CrossRefPubMedGoogle Scholar
  4. 4.
    Giovannini C, Straface E, Modesti D, Coni E, Cantafora A, Vincenzi MD, Malorni W, Masella R (1999) J Nutr 129:1269CrossRefPubMedGoogle Scholar
  5. 5.
    Miró-Casas E, Covas M-I, Fitó M, Farré-Albadalejo M, Marrugat J, Torre R (1999) Eur J Clin Nutr 57:186CrossRefGoogle Scholar
  6. 6.
    Sousa WR, da Rocha C, Cardoso CL, Silva DHS, Zanoni MVB (2004) Food Compos Anal 17:619CrossRefGoogle Scholar
  7. 7.
    Enache TA, Amine A, Brett CMA, Oliveira-Brett AM (2013) Talanta 105:179CrossRefPubMedGoogle Scholar
  8. 8.
    Blekas G, Tsimidou M (2005) Curr Top Nutraceutical Res 3:125Google Scholar
  9. 9.
    Blekas G, Vassilakis C, Harizanis C, Tsimidou M, Boskou DG (2002) J Agric Food Chem 50:3688CrossRefPubMedGoogle Scholar
  10. 10.
    Andjelkovic M, Van Camp J, Trawka A, Verhé R (2010) Eur J Lipid Sci Technol 112:208CrossRefGoogle Scholar
  11. 11.
    Bebeselea A, Manea F, Burtica G, Nagy L, Nagy G (2010) Talanta 80:1068CrossRefPubMedGoogle Scholar
  12. 12.
    Gimenes DT, Cunha RR, Carvalho Ribeiro MM, Pereira PF, Muñoz RAA, Richter EM (2013) Talanta 116:1026CrossRefPubMedGoogle Scholar
  13. 13.
    Bavol D, Economou A, Zima J, Barek J, Dejmkova H (2018) Talanta 178:231CrossRefPubMedGoogle Scholar
  14. 14.
    Tormin TF, Cunha RR, Richter EM, Muñoz RAA (2012) Talanta 99:527CrossRefPubMedGoogle Scholar
  15. 15.
    Surareungchai W, Deepunya W, Tasakorn P (2001) Anal Chim Acta 448:215CrossRefGoogle Scholar
  16. 16.
    Gimenes DT, dos Santos WTP, Tormin TF, Muñoz RAA, Richter EM (2010) Electroanalysis 22:74CrossRefGoogle Scholar
  17. 17.
    Silva WC, Pereira PF, Marra MC, Gimenes DT, Cunha RR, da Silva RAB, Muñoz RAA, Richter EM (2011) Electroanalysis 23:2764CrossRefGoogle Scholar
  18. 18.
    de Miranda JAT, Cunha RR, Gimenes DT, Muñoz RAA, Richter EM (2012) Quim Nova 35:1459CrossRefGoogle Scholar
  19. 19.
    Medeiros RA, Lourenção BC, Rocha-Filho RC, Fatibello-Filho O (2010) Anal Chem 82:8658CrossRefPubMedGoogle Scholar
  20. 20.
    Medeiros RA, Lourenção BC, Rocha-Filho RC, Fatibello-Filho O (2012) Talanta 99:883CrossRefPubMedGoogle Scholar
  21. 21.
    Gimenes DT, dos Santos WTP, Muñoz RAA, Richter EM (2010) Electrochem Commun 12:216CrossRefGoogle Scholar
  22. 22.
    Johnson DC, LaCourse WR (1990) Anal Chem 62:589CrossRefGoogle Scholar
  23. 23.
    Johnson DC, Dobberpuhl D, Roberts R, Vandeberg P (1993) J Chromatogr 640:79CrossRefGoogle Scholar
  24. 24.
    Zima J, Dejmkova H, Barek J (2007) Electroanalysis 19:185CrossRefGoogle Scholar
  25. 25.
    Miller JN, Miller JC (2005) Chemometrics for analytical chemistry, 5th edn. Pearson Education, HarlowGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Analytical Chemistry, Department of ChemistryUniversity of AthensAthensGreece
  2. 2.UNESCO Laboratory of Environmental Electrochemistry, University Research Centre Supramolecular Chemistry, Department of Analytical Chemistry, Faculty of ScienceCharles UniversityPrague 2Czech Republic

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