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Complete genome sequence of a new member of the genus Badnavirus from red pitaya (Hylocereus polyrhizus)


Here, we report a circular double-stranded DNA virus from red pitaya (Hylocereus polyrhizus). The complete genome sequence is 7,837 nt in length and shares 98.7% nucleotide sequence identity with epiphyllum mottle-associated virus (EpMoaV) and 40.4–54.6% with other members of the genus Badnavirus. It has four open reading frames (ORFs), encoding putative proteins of 19.9, 14.8, 225.7 and 14.2 kDa, respectively. The reverse transcriptase (RT)-ribonuclease H (RNase H) region exhibits less than 70.5% nucleotide sequence identity to RT-RNase H of other badnaviruses, and 99.7% to that of EpMoaV. Phylogenetic analysis revealed that the virus from this study and EpMoaV form a single group. Consequently, we propose this virus as a new member of the genus Badnavirus in the family Caulimoviridae and have named it “pitaya badnavirus 1” (PiBV1). PiBV1 and EpMoaV should be considered two isolates of a badnavirus that infects members of the family Cactaceae.

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This research was financially supported by the Youth Fund Program of Guizhou Academy of Agricultural Sciences ([2017]11), the National Natural Science Foundation of China (31560547), and the Guizhou Science and Technology Fund Plan of Guizhou Provincial Department of Science and Technology ([2016]1146).

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Correspondence to Yuhua Ma.

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Zheng, Q., Wang, X., Zhou, J. et al. Complete genome sequence of a new member of the genus Badnavirus from red pitaya (Hylocereus polyrhizus). Arch Virol 165, 749–752 (2020). https://doi.org/10.1007/s00705-019-04503-7

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