Here, we report a circular double-stranded DNA virus from red pitaya (Hylocereus polyrhizus). The complete genome sequence is 7,837 nt in length and shares 98.7% nucleotide sequence identity with epiphyllum mottle-associated virus (EpMoaV) and 40.4–54.6% with other members of the genus Badnavirus. It has four open reading frames (ORFs), encoding putative proteins of 19.9, 14.8, 225.7 and 14.2 kDa, respectively. The reverse transcriptase (RT)-ribonuclease H (RNase H) region exhibits less than 70.5% nucleotide sequence identity to RT-RNase H of other badnaviruses, and 99.7% to that of EpMoaV. Phylogenetic analysis revealed that the virus from this study and EpMoaV form a single group. Consequently, we propose this virus as a new member of the genus Badnavirus in the family Caulimoviridae and have named it “pitaya badnavirus 1” (PiBV1). PiBV1 and EpMoaV should be considered two isolates of a badnavirus that infects members of the family Cactaceae.
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This research was financially supported by the Youth Fund Program of Guizhou Academy of Agricultural Sciences (11), the National Natural Science Foundation of China (31560547), and the Guizhou Science and Technology Fund Plan of Guizhou Provincial Department of Science and Technology (1146).
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Zheng, Q., Wang, X., Zhou, J. et al. Complete genome sequence of a new member of the genus Badnavirus from red pitaya (Hylocereus polyrhizus). Arch Virol 165, 749–752 (2020). https://doi.org/10.1007/s00705-019-04503-7