Complete genomic sequence of crow-dipper mosaic-associated virus, a novel macluravirus infecting Pinellia ternata

  • Rong Wang
  • Bingwei Chen
  • Yong Li
  • Tianyou Wang
  • Wanlong DingEmail author
Annotated Sequence Record


A new macluravirus infecting Pinellia ternata in China was identified by high-throughput sequencing (HTS) and tentatively named “crow-dipper mosaic-associated virus” (CrdMV). The complete genome sequence of CrdMV was determined by reverse transcription (RT) PCR and rapid amplification of cDNA ends (RACE) PCR. The genomic RNA of CrdMV consists of 8,454 nucleotides (nt), excluding the poly(A) tail at the 3′ end. CrdMV has a genomic structure typical of macluraviruses, with large open reading frame encoding a polyprotein of 2,696 amino acids (aa). CrdMV shares 54.40%–59.37% nt sequence identity at the genome sequence level, 48.00%–58.58% aa sequence identity, at the polyprotein sequence level and 37.27%–49.22% aa sequence identity at the CP sequence level with other members of the genus Macluravirus. These values are well below the species demarcation threshold for the family Potyviridae. Phylogenetic analysis based on the amino acid sequences of polyproteins confirmed that CrdMV clusters closely with broad-leafed dock virus A (BDVA, GenBank accession no. KU053507). These results suggest that CrdMV should be considered a distinct member of the genus Macluravirus.



This work was supported by the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences (Grant number 2016-I2M-3-017).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and animal rights

This study did not include experiments with human participants or animals performed by any of the authors.

Supplementary material

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© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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