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Complete genome sequence of rose virus A, the first carlavirus identified in rose

  • Alfredo Diaz-Lara
  • Dimitre Mollov
  • Deborah Golino
  • Maher Al RwahnihEmail author
Annotated Sequence Record
  • 8 Downloads

Abstract

A novel virus was discovered in a Rosa wichuraiana Crep. by high-throughput sequencing and tentatively named “rose virus A” (RVA). Based on sequence identity and phylogenetic analysis, RVA represents a new member of the genus Carlavirus (family Betaflexiviridae). The genome of RVA is 8,849 nucleotides long excluding the poly(A) tail and contains six open reading frames (ORFs). The predicted ORFs code for a replicase, triple gene block (TGB), coat protein, and nucleic acid binding protein, as in a typical carlavirus. RVA is the first carlavirus identified in rose and has the highest nucleotide sequence similarity to poplar mosaic virus. Reverse transcription-PCR-based assays were developed to confirm the presence of RVA in the original source and to screen additional rose plants.

Notes

Acknowledgements

Many thanks to Min Sook Hwang and Kristian Stevens (UC-Davis, Foundation Plant Services) for technical assistance.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animal performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of California-DavisDavisUSA
  2. 2.USDA-ARS, National Germplasm Resources LaboratoryBeltsvilleUSA

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