Analysis of bovine leukemia virus integration sites in cattle under 3 years old with enzootic bovine leukosis

  • Masaki Maezawa
  • Hisashi InokumaEmail author
Brief Report


In the present study, we analyzed bovine leukemia virus (BLV) integration sites in under 3 years old with enzootic bovine leukosis (EBL) cattle and compared these to 30 cattle over 3 years old with EBL. BLV proviruses were integrated near CpG islands and into long interspersed nuclear elements more frequently in EBL cattle under 3 years old than in those over 3 years old. These results suggest that cattle under 3 years old with EBL have different BLV provirus integration sites from those of cattle over 3 years old with EBL, and the BLV provirus integration site may represent one factor contributing to early onset of EBL.



We thank the veterinarians of the Iwate and Tokachi Agriculture Mutual Aid Association and Tokachi and Hayakita Meat Hygiene Inspection Centers for sampling. This work was supported by JSPS KAKENHI grant number 16K15044.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest to declare.

Ethical approval

No ethical approval was necessary, as the present study had no human participants and the authors did not perform any of the procedures on the animals.

Supplementary material

705_2019_4431_MOESM1_ESM.docx (32 kb)
Supplementary material 1 (DOCX 32 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Veterinary MedicineObihiro University of Agriculture and Veterinary MedicineObihiroJapan
  2. 2.United Graduate School of Veterinary SciencesGifu UniversityGifuJapan
  3. 3.Veterinary Medical CenterThe University of TokyoTokyoJapan

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