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Archives of Virology

, Volume 165, Issue 1, pp 227–231 | Cite as

Complete genomic sequence of tea-oil camellia deltapartitivirus 1, a novel virus from Camellia oleifera

  • L.-P. Wu
  • Y.-M. Du
  • H. Xiao
  • L. Peng
  • R. LiEmail author
Annotated Sequence Record
  • 61 Downloads

Abstract

Three viral contig sequences, which represented complete genome of a novel virus with three dsRNAs of 1,712 nucleotides (nt) (dsRNA1), 1,504 nt (dsRNA2) and 1,353 nt (dsRNA3), were found in tea-oil camellia plants by high-throughput sequencing analysis. The three dsRNAs were re-sequenced by RT-PCR cloning. The largest dsRNA, dsRNA1, had a single open reading frame (ORF) that encoded a putative 52.7-kDa protein of a putative viral RNA-dependent RNA polymerase (RdRp). DsRNA2 and dsRNA3 were predicted to encode putative capsid proteins (CPs) of 40.47 kDa and 40.59 kDa, respectively. The virus, which is provisionally named “tea-oil camellia deltapartitivirus 1”,  shared amino acid sequence itentities of 36.09-69.18% with members of the genus Deltapartitivirus on RdRp. Phylogenetic analysis based on RdRp also placed the new virus and other deltapartitiviruses together in a group, suggesting that this virus should be considered a new member of the genus Deltapartitivirus.

Notes

Acknowledgements

This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm” and the National Natural Science Foundation of China (Grant no. 31860525).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any research involving humans or animals.

Supplementary material

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Supplementary material 1 (DOCX 65 kb)
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Supplementary material 5 (DOCX 16 kb)

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Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2019

Authors and Affiliations

  1. 1.School of Life Science, Key Laboratory of Poyang Lake Environment and Resource, Ministry of EducationNanchang UniversityNanchangChina
  2. 2.USDA-ARS, National Germplasm Resources LaboratoryBeltsvilleUSA

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