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Archives of Virology

, Volume 165, Issue 1, pp 215–217 | Cite as

Complete genomic sequence of Pseudomonas lactis bacteriophage HU1 isolated from raw cow’s milk

  • Chikage Tanaka
  • Takahiro Nakayama
  • Takahiro Toba
  • Akiko KashiwagiEmail author
Annotated Sequence Record

Abstract

The lytic cold-active phage HU1, a member of the family Podoviridae, infects Pseudomonas lactis and was first isolated from raw cow’s milk. In this study, we used deep sequencing to determine and analyze the DNA genome sequence of HU1. We identified a 42,551-base-pair genome comprising double-stranded DNA, with 69 predicted open reading frames and a GC content of 56.4%. A whole-genome comparison did not identify HU1 as a member of any previously reported cluster of Pseudomonas phages. By contrast, HU1 was most similar to AF, which infects P. putida, with nucleotide sequence alignment coverage of 24%. These results suggest that HU1 is a novel Pseudomonas phage.

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4423_MOESM1_ESM.pdf (132 kb)
Supplementary material 1 (PDF 131 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Agriculture and Life ScienceHirosaki UniversityHirosakiJapan
  2. 2.Research Institute of Bio-System InformaticsTohoku Chemical Co., Ltd.MoriokaJapan

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