A molecular survey using a validated real-time PCR assay finds no evidence of bovine alphaherpesvirus 2 in samples from animals with suspected vesicular disease in Brazil between 2014 and 2017

  • Felipe Augusto de Souza
  • Mateus Laguardia-Nascimento
  • Marcela Ribeiro Gasparini
  • Luciana Rabello Ferreira
  • Érica Bravo Sales
  • Juliana F. Cargnelutti
  • Marcelo Fernandes Camargos
  • Antônio Augusto Fonseca JúniorEmail author
Brief Report


Bovine alphaherpesvirus 2 (BoHV-2) is the etiologic agent of bovine mammillitis (BM) and pseudo-lumpy skin disease. BM is also important because its clinical presentation can be confused with foot-and-mouth disease (FMD), making it necessary to establish differential diagnoses and perform additional laboratory tests. The objective of this work was to use a validated real-time PCR assay to test for the presence of BoHV-2 in samples from cattle and buffalo with suspected vesicular disease in Brazil. The method could detect the virus at a concentration of 0.5 fg/μL and had 99.4% amplification efficiency, a repeatability error of only 4.1%, and good reproducibility with other reagents. No evidence of BoHV-2 causing vesicular disease in cattle and buffalo was found in this work. This study was able to validate a new methodology for detection of BoHV-2 and evaluate its usefulness for investigating outbreaks of vesicular disease Brazil. The importance of BoHV-2 in cases involving other clinical signs should still be studied using the qPCR developed in this work.



This study was funded by the Brazilian Ministry of Agriculture, Livestock and Supply.

Compliance with ethical standards

Conflict of interest

There are no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Felipe Augusto de Souza
    • 1
  • Mateus Laguardia-Nascimento
    • 2
  • Marcela Ribeiro Gasparini
    • 2
  • Luciana Rabello Ferreira
    • 2
  • Érica Bravo Sales
    • 2
  • Juliana F. Cargnelutti
    • 3
  • Marcelo Fernandes Camargos
    • 2
  • Antônio Augusto Fonseca Júnior
    • 2
    Email author
  1. 1.Centro Universitário Newton PaivaBelo HorizonteBrazil
  2. 2.Laboratório Nacional Agropecuário de Minas Gerais (Lanagro/MG)Pedro LeopoldoBrazil
  3. 3.Universidade Federal de Santa MariaSanta MariaBrazil

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