First identification and molecular characterization of a novel cherry robigovirus
A large contig with sequence similarities to members of the genus Robigovirus was identified by high-throughput sequencing analysis from a symptomless cherry accession. The complete genome sequence of this new virus is 8,384 nucleotides in length, excluding the 3’ poly(A) tail. Its genome organization is very similar to those of four known robigoviruses, encoding a putative replicase, three ‘triple gene block’ proteins, a coat protein, and an unknown protein, 2a. Unlike the four cherry robigoviruses, the new virus does not contain a putative ORF5a. The full-length genome of the virus, which is provisionally named “cherry robigovirus 5” (CRV-5), is 52-57% identical to genome sequences of other robigoviruses. Phylogenetic analysis showed that CRV-5 and other robigoviruses group in a cluster, supporting its assignment to a new species in the genus Robigovirus.
The authors thank Sam Grinstead and Tom Kim for excellent technical assistance. This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm” and the Chinese Scholarship Foundation for Overseas Studies (grant no. 201506825062).
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any research involving humans or animals.
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