First identification and molecular characterization of a novel cherry robigovirus

  • L. Wu
  • H. Liu
  • M. Bateman
  • B. Komorowska
  • R. LiEmail author
Annotated Sequence Record


A large contig with sequence similarities to members of the genus Robigovirus was identified by high-throughput sequencing analysis from a symptomless cherry accession. The complete genome sequence of this new virus is 8,384 nucleotides in length, excluding the 3’ poly(A) tail. Its genome organization is very similar to those of four known robigoviruses, encoding a putative replicase, three ‘triple gene block’ proteins, a coat protein, and an unknown protein, 2a. Unlike the four cherry robigoviruses, the new virus does not contain a putative ORF5a. The full-length genome of the virus, which is provisionally named “cherry robigovirus 5” (CRV-5), is 52-57% identical to genome sequences of other robigoviruses. Phylogenetic analysis showed that CRV-5 and other robigoviruses group in a cluster, supporting its assignment to a new species in the genus Robigovirus.



The authors thank Sam Grinstead and Tom Kim for excellent technical assistance. This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm” and the Chinese Scholarship Foundation for Overseas Studies (grant no. 201506825062).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any research involving humans or animals.

Supplementary material

705_2019_4401_MOESM1_ESM.pptx (95 kb)
Supplementary material 1 (PPTX 94 kb)
705_2019_4401_MOESM2_ESM.docx (21 kb)
Supplementary material 2 (DOCX 21 kb)
705_2019_4401_MOESM3_ESM.txt (8 kb)
Supplementary material 3 (TXT 8 kb)


  1. 1.
    Usenik V, Fabcic J, Stampar F (2008) Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.). Food Chem 107:185–192CrossRefGoogle Scholar
  2. 2.
    Rubio M, Martínez-Gómez P, Marais A, Sánchez-Navarro JA, Pallás V, Candresse T (2017) Recent advances and prospects in Prunus virology. Ann Appl Biol 171:125–138CrossRefGoogle Scholar
  3. 3.
    Zhang YP, Kirkpatrick BC, Smart CD, Uyemoto JK (1998) cDNA cloning and molecular characterization of cherry green ring mottle virus. J Gen Virol 79:2275–2281CrossRefGoogle Scholar
  4. 4.
    Rott ME, Jelkmann W (2001) Complete nucleotide sequence of cherry necrotic rusty mottle virus. Arch Virol 146:395–401CrossRefGoogle Scholar
  5. 5.
    Villamor DEV, Susaimuthu J, Eastwell KC (2015) Genomic analysis of cherry rusty mottle group and cherry twisted leaf-associated viruses reveal a possible new genus within the family Betaflexiviridae. Virol 105:399–408Google Scholar
  6. 6.
    Parker KG, Fridland PR, Gilmer GM (1976) Green ring mottle. Virus diseases and noninfectious diseases of stone fruit in North America. USDA Agric Handbook 437:193–199Google Scholar
  7. 7.
    Liberti D, Ragozzino A, Gentit P, Marais A, Svanella-Dumas L, Candresse T, Llácer G (2004) Biological properties and partial molecular characterization of an apricot strain of CGRMV. Acta Hort 657:103–108CrossRefGoogle Scholar
  8. 8.
    Lee SY, Yea MC, Back CG, Choi KS, Kang IK, Lee SH, Jung HY (2014) Survey of Cherry necrotic rusty mottle virus and cherry green ring mottle virus incidence in Korea by duplex RT-PCR. Plant Pathol J 30:445–449CrossRefGoogle Scholar
  9. 9.
    Zagula KR, Aref NM, Ramsdell DC (1989) Purification, serology, and some properties of a mechanically transmissible virus associated with green ring mottle disease in peach and cherry. Phytopathology 79:451–456CrossRefGoogle Scholar
  10. 10.
    James D, Howell WE, Martin RR (1995) Identification of a flexuous virus associated with cherry twisted leaf disease. Acta Hort 386:86–91CrossRefGoogle Scholar
  11. 11.
    Li RH, Mock R, Hartung Q, Abad J, Hartung SJ, Kinard G (2008) A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J Virol Methods 154:48–55CrossRefGoogle Scholar
  12. 12.
    Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, Cambridge, pp 920–941Google Scholar
  13. 13.
    Morozov SY, Solovyev AG (2003) Triple gene block: modular design of a multifunctional machine for plant virus movement. J Gen Virol 84:1351–1366CrossRefGoogle Scholar

Copyright information

© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply  2019

Authors and Affiliations

  1. 1.National Germplasm Resources LaboratoryUSDA-ARSBeltsvilleUSA
  2. 2.Key Laboratory of Poyang Lake Environment and Resource, School of Life ScienceNanchang UniversityNanchangChina
  3. 3.USDA-APHIS, Plant Protection and QuarantineBeltsvilleUSA
  4. 4.Department of Plant ProtectionResearch Institute of HorticultureSkierniewicePoland

Personalised recommendations