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Complete genome sequence of a divergent strain of potato virus P isolated from Solanum tuberosum in Russia

  • Hironobu YanagisawaEmail author
  • Yosuke Matsushita
  • Aleksandr Khiutti
  • Nina Mironenko
  • Yasuo Ohto
  • Olga Afanasenko
Annotated Sequence Record
  • 13 Downloads

Abstract

Contigs with sequence similarity to potato virus P (PVP), which belongs to the genus Carlavirus, were identified by high-throughput sequencing analysis in potato tubers collected from a farmer’s potato production field in Surazhevka, Artyom, Primorskiy Krai (Russia) in 2018. The complete genome sequence of this virus consisted of 8,394 nucleotides, excluding the poly(A) tail. This is the first report of PVP being detected outside South America. The isolate had high sequence similarity to PVP isolates from Argentina and Brazil, but low sequence similarity was observed in the genes encoding the RNA-dependent RNA polymerase (69% nucleotide sequence identity and 80% amino acid sequence identity) and coat protein (78% nucleotide sequence identity and 89% amino acid sequence identity). Phylogenetic analysis revealed that this PVP-like virus clustered with known PVP isolates but was distinct from them. Comparison of the sequences using the classification criteria of the ICTV indicated that this PVP-like virus is a strain of PVP.

Notes

Acknowledgements

We thank Dr. T. K. Kovalenko, the Head of the Department of Biological Methods of Plant Protection at the Federal State Budget Scientific Institution “Far Eastern Research Institute of Plant Protection”, for helping us sample the potato tubers, and also Y. Narita, J. Sato, and Y. Matsumura for preparing the experimental materials. This study was supported, in part, by the “Pilot Program of International Collaborative Research (Collaborative Research with Russia in Agriculture)” under the “Commissioned Projects for Promotion of Strategic International Collaborative Research for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries” administered by the Ministry of Agriculture, Forestry and Fisheries in Japan.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Ethical approval

This article does not contain any experiments involving humans or animals that have been performed by any of the authors.

Supplementary material

705_2019_4397_MOESM1_ESM.pptx (186 kb)
Supplementary material 1 (PPTX 186 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Hironobu Yanagisawa
    • 1
    Email author
  • Yosuke Matsushita
    • 2
  • Aleksandr Khiutti
    • 3
  • Nina Mironenko
    • 3
  • Yasuo Ohto
    • 1
  • Olga Afanasenko
    • 3
  1. 1.Central Region Agricultural Research CenterNational Agriculture and Food Research OrganizationTsukubaJapan
  2. 2.Institute of Vegetable and Floriculture ScienceNational Agriculture and Food Research OrganizationTsukubaJapan
  3. 3.Federal State Budget Scientific Institution “All-Russian Institute of Plant Protection” (FSBSI VIZR)Saint-PetersburgRussia

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