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Quantification of ovine herpesvirus 2 by digital PCR in an outbreak of malignant catarrhal fever

  • Tatiana Flávia Pinheiro de Oliveira
  • Mateus Laguardia-Nascimento
  • Fabiana Galtarossa Xavier
  • Carla do Amaral Pinto
  • Luciana Rabello Ferreira
  • Ivy de Castro Campos de Souza
  • Márcia Elisa Hammerschmitt
  • Ronaldo Michel Bianchi
  • Júlia Gabriela Wronski
  • Rodrigo Nestor Etges
  • Grazziane Maciel Rigon
  • Marcelo Fernandes Camargos
  • Anselmo Vasconcelos Rivetti Júnior
  • Antonio Augusto Fonseca JuniorEmail author
Brief Report

Abstract

Infection with ovine gammaherpesvirus 2 (OvHV-2) is generally asymptomatic in sheep; however, when it crosses the species barrier, it causes malignant catarrhal fever (MCF) in cattle. In the present study, we developed a real-time PCR assay and a droplet digital PCR assay and use both methods to study an outbreak caused by OvHV-2. Both PCR methods showed high sensitivity and specificity and were able to detect low copy numbers of OvHV-2 in sheep and cattle. The present study describes the first digital PCR quantification of OvHV-2 genome copies in samples collected from sheep and cattle.

Notes

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Tatiana Flávia Pinheiro de Oliveira
    • 1
  • Mateus Laguardia-Nascimento
    • 1
  • Fabiana Galtarossa Xavier
    • 1
  • Carla do Amaral Pinto
    • 1
  • Luciana Rabello Ferreira
    • 1
  • Ivy de Castro Campos de Souza
    • 1
  • Márcia Elisa Hammerschmitt
    • 2
  • Ronaldo Michel Bianchi
    • 2
  • Júlia Gabriela Wronski
    • 2
  • Rodrigo Nestor Etges
    • 3
  • Grazziane Maciel Rigon
    • 3
  • Marcelo Fernandes Camargos
    • 1
  • Anselmo Vasconcelos Rivetti Júnior
    • 1
  • Antonio Augusto Fonseca Junior
    • 1
    Email author
  1. 1.Laboratório Federal de Defesa Agropecuária de Minas GeraisPedro LeopoldoBrazil
  2. 2.Universidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Secretaria da Agricultura, Pecuária e Desenvolvimento RuralPorto AlegreBrazil

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