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Archives of Virology

, Volume 164, Issue 10, pp 2585–2592 | Cite as

Complete genome sequence and phylogenetic analysis of a novel aquareovirus isolated from a diseased marbled eel (Anguilla marmorata)

  • Hui-Wen Huang
  • Chiao-Hsuan Huang
  • Chiu-Ming WenEmail author
Brief Report
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Abstract

Marbled eel reovirus (MERV) is an aquareovirus (AQRV) isolated from diseased marbled eels (Anguilla marmorata) with petechial skin hemorrhage. In this study, we propagated MERV in a cell line derived from the brain of Aequidens rivulatus and purified viral particles by using a discontinuous cesium chloride gradient. Genomic RNA sequences were obtained through next-generation sequencing. MERV, similar to most other AQRVs, showed the presence of 11 double-stranded RNA segments encoding 12 proteins; however, the genome sequence displayed very little similarity to known AQRV sequences. Furthermore, the structural proteins of MERV were most closely related to American grass carp reovirus with sequence identity values of no more than 64.89%. Phylogenetic analysis based on the sequences of structural proteins indicated that MERV shows an evolutionary history between AQRV-B and -G, which belong to the saline and freshwater environment subgroups, respectively. We also observed that MERV showed a closer relationship to orthoreoviruses based on the protein sequences of NS38 and NS73. In summary, MERV is a novel AQRV that could be classified as a member of the new proposed AQRV species “Aquareovirus H”. The taxonomic assignments and evolution of AQRVs thus warrant further investigation.

Notes

Acknowledgements

We acknowledge Wallace Academic Editing for editing this manuscript.

Funding

This study was funded by Ministry of Science and Technology, Taiwan, R.O.C. (grant number MOST105-2313-B-390-001-MY3.).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4365_MOESM1_ESM.csv (31 kb)
Supplementary material 1 (CSV 30 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Life SciencesNational University of KaohsiungKaohsiungTaiwan

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