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Archives of Virology

, Volume 164, Issue 10, pp 2581–2584 | Cite as

Recombinase polymerase amplification assay for rapid detection of maize chlorotic mottle virus in maize

  • Yubing Jiao
  • Junyun Jiang
  • Mengnan An
  • Zihao XiaEmail author
  • Yuanhua WuEmail author
Brief Report
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Abstract

Maize chlorotic mottle virus (MCMV), an important quarantine virus, causes lethal necrosis in maize when coinfected with a potyvirid, which is seriously threatening the production of maize worldwide. In this study, recombinase polymerase amplification (RPA), a novel isothermal DNA amplification and detection technique, was developed to detect MCMV in maize crops. A pair of specific primers was designed based on the conserved sequences of the MCMV coat protein region. The RT-RPA assay was carried out as an isothermal reaction at 38 °C that was complete within 30 min, and no cross-reactivity was detected with other viruses infecting maize in China. The limit of detection of the RT-RPA assay was tenfold lower than that of ordinary RT-PCR. Moreover, this method was successfully applied to test field-collected samples. The newly developed RT-RPA assay offers a reliable, sensitive and efficient method for rapid detection of MCMV in maize in equipment-limited diagnostic laboratories and on-site facilities.

Notes

Acknowledgements

We thank Prof. Zaifeng Fan (China Agricultural University) for providing MCMV, SCMV, RBSDV and PenMV. This research was funded by grants from the Natural Science Foundation of China (31801702).

Author contributions

ZX and YW conceived and designed the experiments. JJ and YJ performed the experiments. JJ and YJ wrote the paper. ZX, MA and YW edited the paper.

Compliance with ethical standards

Conflict of interest

None of the authors have any conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4361_MOESM1_ESM.docx (375 kb)
Supplementary material 1 (DOCX 375 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Plant ProtectionShenyang Agricultural UniversityShenyangChina

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