Archives of Virology

, Volume 164, Issue 10, pp 2617–2620 | Cite as

Molecular characterization of a new trichovirus from peach in Mexico

  • Rodolfo De La Torre-Almaráz
  • Vicente Pallás
  • Jesús A. Sánchez-NavarroEmail author
Annotated Sequence Record


The complete genome sequence of a trichovirus was obtained from peach samples collected from Mexico and found to be 7985 nucleotides long, excluding the poly(A) tail. Phylogenetic analysis using the complete nucleotide sequence revealed that the virus is a member of the genus Trichovirus and is closely related to peach mosaic virus (PcMV) and cherry mottle leaf virus (CMLV). The highest nucleotide sequence identity was 70% to both PcMV and CMLV, indicating that this virus, which we have tentatively named “peach virus M” (PeVM) should be considered a member of a new trichovirus species. We determined, for the first time, the initiation sites of the subgenomic RNAs (sgRNA) of a trichovirus. The sgRNAs for the movement and coat proteins started with the sequence ‘GAA’, while the smallest one, coding for the nucleotide-binding protein, started with the nucleotides ‘GU’. In all cases, the sgRNAs leader ranged between 113 and 121 nt in length.



We thank L. Corachán for her excellent technical assistance.


This work was supported by Grant BIO2017-88321-R from the Spanish Dirección General de Investigación Científica y Técnica (DGICYT) and the Prometeo Program GV2015/010 from the Generalitat Valenciana.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals requiring ethical approval.

Supplementary material

705_2019_4358_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 23 kb)
705_2019_4358_MOESM2_ESM.docx (16 kb)
Supplementary material 2 (DOCX 15 kb)
705_2019_4358_MOESM3_ESM.docx (13 kb)
Supplementary material 3 (DOCX 13 kb)


  1. 1.
    Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Elsevier, San Diego, pp 920–941Google Scholar
  2. 2.
    De La Torre-Almaraz R, Pallas V, Sanchez-Navarro JA (2015) First report of peach latent mosaic viroid in peach trees from Mexico. Plant Dis 99:899CrossRefGoogle Scholar
  3. 3.
    James D, Varga A, Croft H, Rast H, Thompson D, Hayes S (2006) Molecular characterization, phylogenetic relationships, and specific detection of Peach mosaic virus. Phytopathology 96:137–144CrossRefGoogle Scholar
  4. 4.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  5. 5.
    Cambra M, Flores R, Pallas V, Gentit P, Candresse T (2008) Viruses and viroids. In: Layne DR, Bassi D (eds) The peach: botany, production and uses. CAB International, Wallingford, pp 435–466CrossRefGoogle Scholar
  6. 6.
    Maree HJ, Gardner HF, Freeborough MJ, Burger JT (2010) Mapping of the 5’ terminal nucleotides of Grapevine leafroll-associated virus 3 sgRNAs. Virus Res 151:252–255CrossRefGoogle Scholar
  7. 7.
    Sanchez-Navarro JA, Pallas V (1994) Nucleotide-sequence of apple mosaic ilarvirus Rna-4. J Gen Virol 75:1441–1445CrossRefGoogle Scholar
  8. 8.
    Sanchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallas V (2005) Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur J Plant Pathol 111:77–84CrossRefGoogle Scholar
  9. 9.
    Sztuba-Solińska J, Stollar V, Bujarski JJ (2011) Subgenomic messenger RNAs: Mastering regulation of (+)-strand RNA virus life cycle. Virology 412:245–255CrossRefGoogle Scholar
  10. 10.
    Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680CrossRefGoogle Scholar
  11. 11.
    Vives MC, Galipienso L, Navarro L, Moreno P, Guerri J (2002) Characterization of two kinds of subgenomic RNAs produced by citrus leaf blotch virus. Virology 295:328–336CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.UBIPRO, FES-Iztacala, Los Reyes IztacalaTlalnepantla de BazMexico
  2. 2.Instituto de Biología Molecular y Celular de Plantas, IBMCP (Consejo superior de Investigaciones Científicas-Universidad Politécnica de Valencia)ValenciaSpain

Personalised recommendations