Molecular characterization of a new trichovirus from peach in Mexico
The complete genome sequence of a trichovirus was obtained from peach samples collected from Mexico and found to be 7985 nucleotides long, excluding the poly(A) tail. Phylogenetic analysis using the complete nucleotide sequence revealed that the virus is a member of the genus Trichovirus and is closely related to peach mosaic virus (PcMV) and cherry mottle leaf virus (CMLV). The highest nucleotide sequence identity was 70% to both PcMV and CMLV, indicating that this virus, which we have tentatively named “peach virus M” (PeVM) should be considered a member of a new trichovirus species. We determined, for the first time, the initiation sites of the subgenomic RNAs (sgRNA) of a trichovirus. The sgRNAs for the movement and coat proteins started with the sequence ‘GAA’, while the smallest one, coding for the nucleotide-binding protein, started with the nucleotides ‘GU’. In all cases, the sgRNAs leader ranged between 113 and 121 nt in length.
We thank L. Corachán for her excellent technical assistance.
This work was supported by Grant BIO2017-88321-R from the Spanish Dirección General de Investigación Científica y Técnica (DGICYT) and the Prometeo Program GV2015/010 from the Generalitat Valenciana.
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Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals requiring ethical approval.
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