Advertisement

Archives of Virology

, Volume 164, Issue 10, pp 2573–2580 | Cite as

Detection of new vitiviruses infecting grapevine in California

  • Alfredo Diaz-Lara
  • Reid S. Brisbane
  • Kamyar Aram
  • Deborah Golino
  • Maher Al RwahnihEmail author
Brief Report
  • 119 Downloads

Abstract

Recently, five new viruses from the genus Vitivirus were identified and named grapevine virus G, H, I, J and L. These viruses were targeted in a survey to evaluate their prevalence in different grapevine populations in California. Excluding a single detection of GVJ, other vitiviruses were detected infecting several grapevine selections via RT-PCR and later confirmed by sequencing. This paper represents the first report of GVG, GVH and GVI in California. In a preliminary analysis, the sequence diversity between identified isolates of GVG, GVH, GVI and GVL was investigated using distance matrices and phylogenetics. Finally, coinfections involving diverse vitiviruses and leafroll viruses were evidenced.

Notes

Acknowledgements

Thanks to B. Prins for providing the samples from the USDA National Clonal Germplasm Repository and thanks to Arnaud G. Blouin (The New Zealand Institute for Plant and Food Research Limited) for providing positive controls for GVG and GVI.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animal performed by any of the authors.

Supplementary material

705_2019_4355_MOESM1_ESM.pptx (333 kb)
Supplementary material 1 (PPTX 333 kb)
705_2019_4355_MOESM2_ESM.xlsx (20 kb)
Supplementary material 2 (XLSX 20 kb)
705_2019_4355_MOESM3_ESM.doc (66 kb)
Supplementary material 3 (DOC 65 kb)
705_2019_4355_MOESM4_ESM.docx (13 kb)
Supplementary material 4 (DOCX 13 kb)
705_2019_4355_MOESM5_ESM.xlsx (34 kb)
Supplementary material 5 (XLSX 34 kb)

References

  1. 1.
    Dolja VV, Meng B, Martelli GP (2017) Evolutionary aspects of grapevine virology. Grapevine viruses: molecular biology, diagnostics and management. Springer, Cham, pp 659–688CrossRefGoogle Scholar
  2. 2.
    Minafra A, Mawassi M, Goszczynski D, Saldarelli P (2017) Grapevine vitiviruses. Grapevine viruses: molecular biology, diagnostics and management. Springer, Cham, pp 229–256CrossRefGoogle Scholar
  3. 3.
    Herrbach E, Alliaume A, Prator CA et al (2017) Vector transmission of grapevine leafroll-associated viruses. In: Meng B, Martelli GP, Golino DA, Fuchs M (eds) Grapevine viruses: molecular biology, diagnostics and management. Springer International Publishing, Cham, pp 483–503CrossRefGoogle Scholar
  4. 4.
    Martelli GP, Minafra A, Saldarelli P (1997) Vitivirus, a new genus of plant viruses. Arch Virol 142:1929–1932Google Scholar
  5. 5.
    Blouin AG, Keenan S, Napier KR et al (2017) Identification of a novel vitivirus from grapevines in New Zealand. Arch Virol.  https://doi.org/10.1007/s00705-017-3581-0 Google Scholar
  6. 6.
    Candresse T, Theil S, Faure C, Marais A (2017) Determination of the complete genomic sequence of grapevine virus H, a novel vitivirus infecting grapevine. Arch Virol.  https://doi.org/10.1007/s00705-017-3587-7 Google Scholar
  7. 7.
    Blouin AG, Chooi KM, Warren B et al (2018) Grapevine virus I, a putative new vitivirus detected in co-infection with grapevine virus G in New Zealand. Arch Virol.  https://doi.org/10.1007/s00705-018-3738-5 Google Scholar
  8. 8.
    Diaz-Lara A, Golino D, Al Rwahnih M (2018) Genomic characterization of Grapevine virus J, a novel virus identified in grapevine. In: Proceedings of the 19th Congress of ICVG, Santiago, pp 168–169Google Scholar
  9. 9.
    Debat HJ, Zavallo D, Gomez-Talquenca S, Asurmendi S (2018) Evidence of a novel vitivirus in grapevine cv Riesling. bioRxiv.  https://doi.org/10.1101/314674 Google Scholar
  10. 10.
    Debat HJ, Zavallo D, Brisbane RS et al (2018) Grapevine virus L: a novel vitivirus in grapevine. bioRxiv.  https://doi.org/10.1101/314674 Google Scholar
  11. 11.
    Puckett J, Al Rwahnih M, Klassen V, Golino D (2018) The Davis grapevine virus collection—a current perspective. In: Proceedings of the 19th congress of ICVG, Santiago, pp 186–187Google Scholar
  12. 12.
    Osman F, Rowhani A (2006) Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan). J Virol Methods 133:130–136.  https://doi.org/10.1016/j.jviromet.2005.11.005 CrossRefGoogle Scholar
  13. 13.
    Sharma AM, Wang J, Duffy S et al (2011) Occurrence of grapevine leafroll-associated virus complex in Napa Valley. PLoS One 6:e26227.  https://doi.org/10.1371/journal.pone.0026227 CrossRefGoogle Scholar
  14. 14.
    Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797.  https://doi.org/10.1093/nar/gkh340 CrossRefGoogle Scholar
  15. 15.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874.  https://doi.org/10.1093/molbev/msw054 CrossRefGoogle Scholar
  16. 16.
    Sciancalepore A, Pio Ribeiro G, Turturo C et al (2006) Molecular variability of GVA and GVB coat protein genes in naturally infected grapevine accessions. In: Extended abstracts of the 15th meeting of ICVG, Stellenbosch, pp 81–82Google Scholar
  17. 17.
    Rosa C, Jimenez JF, Margaria P, Rowhani A (2011) Symptomatology and effects of viruses associated with rugose wood complex on growth of four different rootstocks. Am J Enol Vitic.  https://doi.org/10.5344/ajev.2011.10104 Google Scholar
  18. 18.
    Osman F, Leutenegger C, Golino D, Rowhani A (2007) Real-time RT-PCR (TaqMan®) assays for the detection of Grapevine Leafroll associated viruses 1–5 and 9. J Virol Methods 141:22–29.  https://doi.org/10.1016/j.jviromet.2006.11.035 CrossRefGoogle Scholar
  19. 19.
    Diaz-Lara A, Klaassen V, Stevens K et al (2018) Characterization of grapevine leafroll-associated virus 3 genetic variants and application towards RT-qPCR assay design. PLoS One 13:e0208862.  https://doi.org/10.1371/journal.pone.0208862 CrossRefGoogle Scholar
  20. 20.
    Rowhani A, Daubert S, Arnold K et al (2018) Synergy between grapevine vitiviruses and grapevine leafroll viruses. Eur J Plant Pathol 151:919–925.  https://doi.org/10.1007/s10658-018-1426-7 CrossRefGoogle Scholar
  21. 21.
    Alabi OJ, McBride S, Appel DN et al (2019) Grapevine virus M, a novel vitivirus discovered in the American hybrid bunch grape cultivar Blanc du Bois in Texas. Arch Virol.  https://doi.org/10.1007/s00705-019-04252-7 Google Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Alfredo Diaz-Lara
    • 1
  • Reid S. Brisbane
    • 2
  • Kamyar Aram
    • 1
  • Deborah Golino
    • 1
  • Maher Al Rwahnih
    • 1
    Email author
  1. 1.Department of Plant PathologyUniversity of California-DavisDavisUSA
  2. 2.Foundation Plant ServicesUniversity of California-DavisDavisUSA

Personalised recommendations