Archives of Virology

, Volume 164, Issue 10, pp 2627–2630 | Cite as

Genome characterization of the novel lytic Vibrio parahaemolyticus phage vB_VpP_BA6

  • Meiyan Yang
  • Yongjian Liang
  • Runbin Su
  • Hanfang Chen
  • Jing Wang
  • Jumei Zhang
  • Yu Ding
  • Li Kong
  • Haiyan Zeng
  • Liang Xue
  • Haoming Wu
  • Qingping WuEmail author
Annotated Sequence Record


A lytic bacteriophage, designated Vibrio phage vB_VpP_BA6, was isolated from sewage collected in Guangzhou, China. The double-stranded DNA genome of phage BA6 is composed of 50,520 bp with a G+C content of 41.77%. It possesses 64 open reading frames relating to phage structure, packaging, host lysis, DNA metabolism, and additional functions. Three tRNAs genes (encoding Pro, Ile and Trp) were detected. Comparison of its genomic features and phylogenetic analysis revealed that phage BA6 is a novel member of the family Podoviridae. This phage may represent a potential therapeutic agent against multidrug-resistant Vibrio parahaemolyticus.



The authors are grateful to the National Key R&D Program of China (2017YFC1601201), the GDAS’ Project of Science and Technology Development (No. 2019GDASYL-0103011), the Key Project of Natural Science Foundation of China (31730070) and GDAS’ Special Project of Science and Technology Development (2017GDASCX-0201) for financial support.

Supplementary material

705_2019_4351_MOESM1_ESM.tif (2 mb)
Supplementary Fig. S1 Transmission electron microscopy image of vB_VpP_BA6
705_2019_4351_MOESM2_ESM.tif (3.4 mb)
Supplementary Fig. S2 Terminal sequence determination in the vB_VpP_BA6 genome. The panels show the run-off sequencing spectrograms of the phage vB_VpP_BA6 genome using a forward primer (Forward: 5’-CTGGTGGAAGAACT GTAGAA-3′) and a reverse primer (Reverse: 5’-ATACCCGTTGCTTATCATC-3’). (A) The 3’-terminal sequence. (B) The 5’-terminal sequence
705_2019_4351_MOESM3_ESM.xlsx (10 kb)
Supplementary Table S1 Lytic spectra of vB_VpP_BA6 based on 30 bacterial strains of Vibrio parahaemolyticus. Clear lysis zone (+), no lysis zone (−)
705_2019_4351_MOESM4_ESM.xlsx (23 kb)
Supplementary Table S2 Open reading frames in the phage vB_VpP_BA6 genome


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Meiyan Yang
    • 1
    • 2
  • Yongjian Liang
    • 1
    • 2
  • Runbin Su
    • 2
  • Hanfang Chen
    • 2
  • Jing Wang
    • 2
  • Jumei Zhang
    • 1
  • Yu Ding
    • 3
  • Li Kong
    • 1
  • Haiyan Zeng
    • 1
  • Liang Xue
    • 1
  • Haoming Wu
    • 1
  • Qingping Wu
    • 1
    Email author
  1. 1.State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Open Laboratory of Applied MicrobiologyGuangdong Institute of MicrobiologyGuangzhouChina
  2. 2.College of AgricultureSouth China Agricultural UniversityGuangzhouChina
  3. 3.Department of Food Science and TechnologyJinan UniversityGuangzhouChina

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