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Nyctinomops laticaudatus bat-associated Rabies virus causes disease with a shorter clinical period and has lower pathogenic potential than strains isolated from wild canids

  • Natalia Langenfeld Fuoco
  • Elaine Raniero Fernandes
  • Fernanda Guedes
  • Sandriana Dos Ramos Silva
  • Leticia Patricia Guimarães
  • Nayara Ugeda Silva
  • Orlando Garcia Ribeiro
  • Iana Suly Santos KatzEmail author
Original Article
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Abstract

Rabies is a lethal viral disease that can affect a wide range of mammals. Currently, Rabies virus (RABV) in some European and American countries is maintained primarily in wild species. The regulation of viral replication is one of the critical mechanisms involved in RABV pathogenesis. However, the relationship between replication and the pathogenesis of RABV isolated from wild animals remains poorly understood. In the present study, we evaluated the pathogenicity of the street viruses Nyctinomops laticaudatus bat-associated RABV (NYBRV) and Cerdocyon thous canid-associated RABV (CECRV). Infection of mice with NYBRV led to 33% mortality with rapid disease evolution and marked histopathological changes in the CNS. In contrast, infection with CECRV led to 67% mortality and caused mild neuropathological lesions. The proportion of RABV antigen was significantly higher in the cytoplasm of neuronal cells of the cerebral cortex and in the meninges of mice infected with CECRV and NYBRV, respectively. Moreover, the replication rate of NYBRV was significantly higher (p < 0.001) than that of CECRV in neuroblastoma cells. However, CECRV replicated to a significantly higher titer in epithelial cells. Our results indicate that NYBRV infection results in rapid disease progression accompanied by frequent and intense histopathological alterations in the CNS in mice, and in a high replication rate in neuroblastoma cells. Although, CECRV is more pathogenic in mice, it caused milder histopathological changes in the CNS and replicated more efficiently in epithelial cells. Our data point to a correlation between clinical aspects of disease and the replication of RABV in different cell lines.

Notes

Acknowledgements

We would like to thank Karen Myiuki Asano at the Pasteur Institute for providing the mice. This work was supported by Instituto Pasteur, São Paulo, Brazil.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Animal rights statement

The study protocol was approved by the Committee on the Ethics of Animal Experiments of the Instituto Pasteur of São Paulo. All institutional and national guidelines for the care and use of laboratory animals were followed.

Consent for publication

All authors consent to publication.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Natalia Langenfeld Fuoco
    • 1
  • Elaine Raniero Fernandes
    • 1
  • Fernanda Guedes
    • 1
  • Sandriana Dos Ramos Silva
    • 1
  • Leticia Patricia Guimarães
    • 1
  • Nayara Ugeda Silva
    • 1
  • Orlando Garcia Ribeiro
    • 2
  • Iana Suly Santos Katz
    • 1
    Email author
  1. 1.Pasteur InstituteSão PauloBrazil
  2. 2.Laboratory of ImmunogeneticsButantan InstituteSão PauloBrazil

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