First detection and genetic characterization of peste des petits ruminants virus from dorcas gazelles “Gazella dorcas” in the Sudan, 2016-2017
- 43 Downloads
In May 2017, many free-ranging dorcas gazelles (Gazella dorcas) with suspected signs of peste des petits ruminants (PPR) were reported in Dinder National Park, South-Eastern Sudan. Peste des petits ruminants virus (PPRV) antigen and nucleic acid were detected in specimens from these gazelles using an immunocapture ELISA and a reverse transcription polymerase chain reaction (RT-PCR) assays. PPRV was also detected in four healthy semi-captive dorcas gazelles from two areas of Khartoum State. Phylogenetic analysis showed that these PPRV strains belonged to the lineage IV genotype. The present study demonstrates that gazelles are a potential wild small ruminant host for PPRV and may influence the epidemiology of PPR in the Sudan.
We are grateful to Prof. Elsayed Elowni (University of Khartoum) and Prof. Satya Parida (The Pirbright Institute, UK) for their valuable comments, the staff of the General Directorate of the Epizootics Disease Control, Khartoum, Sudan, and the Kuku Zoo (Sudan University of Science and Technology) for their kind assistance with collection of samples.
RMA performed most of the laboratory work and data analysis. ML helped with data interpretation and revised and finalized the manuscript. SA, WHA, BAM and SMM also contributed to these laboratory studies. AB (deceased) also contributed to the design of this study and laboratory work. NAO was responsible for the design and supervision of this research project, data analysis and interpretation, sequence and phylogenetic analysis, and wrote and finalized the drafted manuscript.
The research in this study was partially funded by a project from the Food and Agriculture Organization (FAO) (Grant No. SUD/TCP/3504) in collaboration with the Central Veterinary Research Laboratory (CVRL), Soba, Khartoum, Sudan, and by a research project for N.A. Osman from the Republic of the Sudan, Sudanese Ministry of Higher Education and Scientific Research, Commission of Scientific Research and Innovation (Grant No. 2017/972).
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
- 1.Gargadennec L, Lalanne A (1942) La peste des petits ruminants. Bulletin des Services Zootechniques et des Epizooties de L’Afrique Occidental Français 5(1):16–21Google Scholar
- 4.OIE-WAHIS (2018) Peste des petits ruminants, Bulgaria. Annual Animal Health Report, World Animal Health Information Database (WAHIS Interface)—Version 1, World Organisation for Animal Health (OIE). http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?reportid=27029
- 5.OIE-FAO (2015) Global strategy for the control and eradication of PPR. World Organization for Animal Health (OIE); Food and Agriculture Organization of the United Nations (FAO), Contract No.: ISBN 978-92-9044-989-8; ISBN 978-92-5-108733-6. http://www.fao.org/3/a-i4460e.pdf
- 7.Elhag Ali B (1973) A natural outbreak of rinderpest involving sheep, goats and cattle in Sudan. Bull Epizoot Dis Afr 21:421–428Google Scholar
- 15.Diallo A, Taylor WP, Lefèvre PC, Provost A (1989) Atténuation d’une souche de virus de la peste des petits ruminants: candidat pour un vaccin homologue vivant. Revue d’élevage et de médecine vétérinaire des pays tropicaux 42(3):311–319Google Scholar
- 20.Sharawi SS, Yousef MR, Al-Hofufy AN, Al-Blowi MH (2010) Isolation, serological and real time PCR diagnosis of peste des petites ruminants virus in naturally exposed Arabian Gazelle in Saudi Arabia. Vet World 3(11):489–494Google Scholar