Archives of Virology

, Volume 164, Issue 10, pp 2593–2597 | Cite as

Complete genome sequence and analysis of a new lethal arterivirus, Trionyx sinensis hemorrhagic syndrome virus (TSHSV), amplified from an infected Chinese softshell turtle

  • Sunjian Lyu
  • Xuemei Yuan
  • Haiqi Zhang
  • Weida Shi
  • Xiaoying Hang
  • Li LiuEmail author
  • Zheng Cao
  • Yinglei Wu
Annotated Sequence Record


Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a newly discovered lethal arterivirus that causes serious disease in Trionyx sinensis in China. In this study, the complete genome sequence of TSHSV was determined by RACE cloning, and the functions of the predicted proteins were predicted. The complete genome of TSHSV was found to be 17,875 bp in length, and a 3’-end poly(A) tail was detected. Eight TSHSV hypothetical proteins (TSHSV-HPs) were predicted by gene model identification. TSHSV-HP2, 3 and 4 were associated with replicase activity, since papain-like protease (PLPs), serine-type endopeptidase, P-loop-containing nucleoside triphosphate hydrolase, and EndoU-like endoribonuclease motifs were detected. Phylogenetic analysis showed that TSHSV clusters with an arterivirus from a Chinese broad-headed pond turtle.



We would like to thank the Huzhou Fisheries Technical Extension Station for providing ill T. sinensis.


This study was partially funded by the Zhejiang Provincial Science and Technology Program (Project no. 2018F10013) and the Zhejiang Provincial Natural Science Foundation (Project no. LY15C190001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors. Samples were obtained from a T. sinensis farm, and this study did not include animal experiments.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang ProvinceZhejiang Institute of Freshwater FisheriesHuzhouChina

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