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A virome from ornamental flowers in an Australian rural town

  • S. J. WylieEmail author
  • T. T. Tran
  • D. Q. Nguyen
  • S.-H. Koh
  • A. Chakraborty
  • W. Xu
  • M. G. K. Jones
  • H. Li
Original Article
  • 56 Downloads

Abstract

Samples of leaves exhibiting symptoms resembling those caused by virus infection were collected from ornamental street flowers in a rural town in Western Australia. Thirty-seven leaf samples were collected from plants of iris, tulip, lily, daffodil, stock and grape hyacinth. Shotgun sequencing of cDNA derived from leaf samples was done, and analysis showed that about 6% of the sequences obtained were of viral origin. Assembly of virus-like sequences revealed complete or partial genome sequences of 13 virus isolates representing 11 virus species. Eight of the isolates were of potyviruses, one was of a macluravirus, three were of potexviruses, and one was of a bunya-like virus. The complete genome of an isolate originally classified as ornithogalum mosaic virus was genetically divergent and differed in polyprotein cleavage motifs, and we propose that this isolate represents a distinct species. The implications of importing to Australia live plant propagules infected with viruses are discussed.

Notes

Acknowledgements

We acknowledge Mr. Steve Winfield, Head Gardener of the Shire of Nannup, Western Australia, for his assistance. Tran TT and Nguyen DQ each received a scholarship provided jointly by Vietnam International Education Development (VIED) and Murdoch University. Xu W received a scholarship provided jointly by Jiangsu Academy of Agricultural Sciences (JAAS) and Murdoch University.

Compliance with ethical standards

Conflicts of interest

The authors disclose no conflicts of interest.

Research involving human participants and/or animals

This research was carried out without involving human participants and/or animal subjects.

Informed consent

This research was carried out with the fully informed consent of all parties.

Supplementary material

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Supplementary material 1 (TXT 95 kb)
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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • S. J. Wylie
    • 1
    Email author
  • T. T. Tran
    • 1
  • D. Q. Nguyen
    • 1
  • S.-H. Koh
    • 1
  • A. Chakraborty
    • 1
  • W. Xu
    • 1
    • 2
  • M. G. K. Jones
    • 1
  • H. Li
    • 1
  1. 1.Plant Biotechnology Research Group-Virology, Western Australian State Agricultural Biotechnology Centre, School of Veterinary and Life SciencesMurdoch UniversityMurdochAustralia
  2. 2.Jiangsu Academy of Agricultural SciencesNanjingChina

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