Development of sensitive molecular assays for the detection of grapevine leafroll-associated virus 3 in an insect vector
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Grapevine leafroll-associated virus 3 (GLRaV-3) is an economically significant virus of grapevines, with secondary spread mediated by several species of mealybug and soft scale insects. To better understand virus-vector interactions, sensitive virus detection in these insects is a key tool. In this research, two new hydrolysis-probe-based real-time assays for GLRaV-3 detection were developed and compared to three existing assays. Of the five assays compared, the one-step RT-qPCR probe-based assay was the most sensitive and reliable, with as few as 10 virus RNA copies detected. This is the first description of a real-time molecular assay for virus detection in mealybugs with such sensitivity.
The authors are grateful for financial support from the Bio-protection Research Centre and helpful critique of the manuscript by Joanna Bowen and Kieren Arthur.
Compliance with ethical standards
This study was funded by the Bio-protection Research Centre.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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