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Development of sensitive molecular assays for the detection of grapevine leafroll-associated virus 3 in an insect vector

  • Brogan McGrealEmail author
  • Manoharie Sandanayaka
  • Kar Mun Chooi
  • Robin MacDiarmid
Brief Report
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Abstract

Grapevine leafroll-associated virus 3 (GLRaV-3) is an economically significant virus of grapevines, with secondary spread mediated by several species of mealybug and soft scale insects. To better understand virus-vector interactions, sensitive virus detection in these insects is a key tool. In this research, two new hydrolysis-probe-based real-time assays for GLRaV-3 detection were developed and compared to three existing assays. Of the five assays compared, the one-step RT-qPCR probe-based assay was the most sensitive and reliable, with as few as 10 virus RNA copies detected. This is the first description of a real-time molecular assay for virus detection in mealybugs with such sensitivity.

Notes

Acknowledgements

The authors are grateful for financial support from the Bio-protection Research Centre and helpful critique of the manuscript by Joanna Bowen and Kieren Arthur.

Compliance with ethical standards

Funding

This study was funded by the Bio-protection Research Centre.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4310_MOESM1_ESM.doc (54 kb)
Supplementary material 1 (DOC 54 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.The New Zealand Institute for Plant and Food Research LimitedAucklandNew Zealand
  2. 2.School of Biological SciencesThe University of AucklandAucklandNew Zealand

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