Molecular characterization and detection of two carlaviruses infecting cactus
Two large contigs with sequence similarities to different carlaviruses were identified by high-throughput sequencing in samples from a cactus plant. The complete genomes of the two viruses, tentatively named “cactus carlavirus 1” (CCV-1) and “cactus carlavirus 2” (CCV-2), were determined to be 8,441 and 8,396 nucleotides long, respectively, excluding the poly(A) tail. These viruses have the typical genomic organization of members of the genus Carlavirus. CCV-1 appears to be a cactus isolate of the carlavirus HSO-2016a, with 90.1% nucleotide sequence identity between the two virus genomes, whereas CCV-2 may be classified as a member of a new species. The sequences of CCV-2 and other carlaviruses are 48.9-60.0% identical at the whole-genome level.
This study was supported by the National Natural Science Foundation of China (Grant no. 31860525) and the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D, “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm”. The authors thank Mathew Buhl and Meya Ngundam for their technical assistance.
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Conflict of interest
All authors declare that they have no conflict of interest.
This article does not contain any research involving humans or animals.
- 1.Adams MJ, Lefkowitz EJ, King AMQ, Harrach B, Harrison RL, Knowles NJ, Kropinski AM, Krupovic M, Kuhn JH, Mushegian AR, Nibert M, Sabanadzovic S, Sanfacon H, Siddell SG, Simmonds P, Varsani A, Zerbini FM, Gorbalenya AE, Davison AJ (2017) Changes to taxonomy and the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses. Arch Virol 162:2505–2538CrossRefGoogle Scholar
- 3.Kreuze J, Hammond J, Pearson MN, Martelli GP, Adams MJ, Saldarelli P, Ryu KH, Namba S, Candresse T, Yoshikawa N (2012) Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, London, pp 920–941Google Scholar
- 4.Stevens PF (2001) Angiosperm phylogeny website version 14Google Scholar
- 7.Morris SJ (1977) Viruses of Opuntia spp. Zeitschrift fuer pflanzenkrankheiten und pflanzenschutz 84:468–472Google Scholar
- 10.Park CH, Song EG, Ryu KH (2018) Detection of co-infection of Notocactus leningausii f. Cristatus with six virus species in South Korea. Plant Pathol J 34:65–70Google Scholar