Archives of Virology

, Volume 164, Issue 7, pp 1873–1876 | Cite as

Molecular characterization and detection of two carlaviruses infecting cactus

  • L. Peng
  • S. Grinstead
  • G. Kinard
  • L.-P. WuEmail author
  • R. LiEmail author
Brief Report


Two large contigs with sequence similarities to different carlaviruses were identified by high-throughput sequencing in samples from a cactus plant. The complete genomes of the two viruses, tentatively named “cactus carlavirus 1” (CCV-1) and “cactus carlavirus 2” (CCV-2), were determined to be 8,441 and 8,396 nucleotides long, respectively, excluding the poly(A) tail. These viruses have the typical genomic organization of members of the genus Carlavirus. CCV-1 appears to be a cactus isolate of the carlavirus HSO-2016a, with 90.1% nucleotide sequence identity between the two virus genomes, whereas CCV-2 may be classified as a member of a new species. The sequences of CCV-2 and other carlaviruses are 48.9-60.0% identical at the whole-genome level.



This study was supported by the National Natural Science Foundation of China (Grant no. 31860525) and the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D, “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm”. The authors thank Mathew Buhl and Meya Ngundam for their technical assistance.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any research involving humans or animals.

Supplementary material

705_2019_4279_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 14 kb)
705_2019_4279_MOESM2_ESM.txt (17 kb)
Supplementary material 2 (TXT 17 kb)


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Poyang Lake Environment and Resource, School of Life ScienceNanchang UniversityNanchangChina
  2. 2.USDA-ARS, National Germplasm Resources LaboratoryBeltsvilleUSA

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