Faba bean polerovirus 1 (FBPV-1); a new polerovirus infecting legume crops in Australia
- 12 Downloads
A new polerovirus species with the proposed name faba bean polerovirus 1 (FBPV-1) was found in winter legume crops and weeds in New South Wales, Australia. We describe the complete genome sequence of 5,631 nucleotides, containing all putative open reading frames, from two isolates, one from faba bean (Vicia faba) and one from chickpea (Cicer arietinum). FBPV-1 has a genome organization typical of poleroviruses with six open reading frames. However, recombination analysis strongly supports a recombination event in which the 5′ portion of FBPV-1, which encodes for proteins P0, P1 and P1-P2, appears to be from a novel parent with a closest nucleotide identity of only 66% to chickpea chlorotic stunt virus. The 3′ portion of FBPV-1 encodes for proteins P3, P4 and P3-P5 and shares 94% nucleotide identity to a turnip yellows virus isolate from Western Australia.
This study was funded by Australian Grains Research and Development Corporation projects DAQ00154, DAQ00186 and DAN00202.
Compliance with ethical standards
Conflict of interest
The authors declare there are no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 2.Abraham AD, Varrelmann M, Vetten HJ (2008) Molecular evidence for the occurrence of two new luteoviruses in cool season food legumes in Northeast Africa. Afr J Biotechnol 7:414–420Google Scholar
- 6.Bushnell B (2016) BBTools: a suit of bioinformatic tools used for DNA and RNA sequence data analysis. http://jgi.doe.gov/data-and-tools/bbtools/. Accessed 23 May 2016
- 11.Herrbach E (1999) Vector-virus interaction. In: Smith HG, Barker H (eds) The luteoviridae. CAB International, Wallingford, pp 85–146Google Scholar
- 14.Katul L (1992) Characterization by serology and molecular biology of bean leaf roll virus and faba bean necrotic yellows virus. PhD thesis, University of Göttingen, Göttingen, Germany, p 115Google Scholar
- 15.King AM, Lefkowitz E, Adams MJ, Carstens EB (2011) Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Elsevier, San DiegoGoogle Scholar
- 16.Kozlowska-Makulska A, Hasiow-Jaroszewska B, Szyndel M, Herrbach E, Bouzoubaa S, Lemaire O, Beuve M (2015) Phylogenetic relationships and the occurrence of interspecific recombination between beet chlorosis virus (BChV) and Beet mild yellowing virus (BMYV). Arch Virol 160:429–433CrossRefGoogle Scholar
- 22.Mayo MA, Miller WA (1999) The structure and expression of luteovirus genomes. In: Smith HG, Barker H (eds) The luteoviridae. CAB International, Wallingford, pp 23–42Google Scholar
- 24.Najar A, Kumari SG, Attar N, Lababidi S (2011) Present status of some virus diseases affecting legume crops in Tunisia, and partial characterization of Chickpea chlorotic stunt virus. Phytopathol Mediterr 50:310–315Google Scholar
- 33.van den Heuvel JF, Bruyère A, Hogenhout SA, Ziegler-Graff V, Brault V, Verbeek M, van der Wilk F, Richards K (1997) The N-terminal region of the luteovirus readthrough domain determines virus binding to Buchnera GroEL and is essential for virus persistence in the aphid. J Virol 71:7258–7265Google Scholar
- 35.Ziegler-Graff V, Brault V, Mutterer JD, Simonis MT, Herrbach E, Guilley H, Richards KE, Jonard G (1996) The coat protein of beet western yellows luteovirus is essential for systemic infection but the viral gene products P29 and P19 are dispensable for systemic infection and aphid transmission. Mol Plant Microbe Interact 9(6):501–510CrossRefGoogle Scholar