First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China

  • Chao Ye
  • Qingyuan Huang
  • Tingting Chen
  • Jiali Jiang
  • Fengqing Hou
  • Dongyi Xu
  • Yuanyi Peng
  • Rendong FangEmail author
  • Jing ChenEmail author
Brief Report


Enzootic nasal adenocarcinoma (ENA) of goats, characterized by transformation of epithelial cells of the ethmoid turbinates, is caused by enzootic nasal tumor virus 2 (ENTV-2). ENTV-2 belongs to the genus Betaretrovirus and has extended its distribution globally with a high prevalence; however, the genetic diversity and genotypic distribution for ENTV-2 have not been analyzed systematically due to the limited availability of sequence data. In this study, an infection by ENTV-2 was detected by RT-PCR in Chongqing in July 2018, and the complete sequence of one strain (CQ1) was determined. Phylogenetic analysis indicated a high degree of genetic heterogeneity among ENTV-2 sequences, with the existence of two main lineages. Lineage 1 and 2 were composed of ENTV-2 from China and the UK, respectively. Although CQ1 was closely related to recent ENTV-2 strains collected in the neighboring provinces of Chongqing (Shaanxi and Sichuan), it formed a separate sublineage of lineage 1 (sublineage 1.3). This report will enhance our understanding of the epidemiology of ENTV-2 in China.


Enzootic nasal tumor virus 2 Virus detection Chongqing Genotypic analysis Lineages 



This work was supported by the National Key Research and Development Program of China (2018YFD0500500), the Chongqing Science & Technology Commission (cstc2018jscx-msybX0302, cstc2018jscx-msybX0221), the earmarked fund for China Agriculture Research System (CARS-37), the Fundamental Research Funds for the Central Universities (XDJK2017A003, SWU118072), and the Innovation Team Building Program in Chongqing Universities (CXTDG201602004).

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

705_2019_4211_MOESM1_ESM.docx (116 kb)
Supplementary material 1 (DOCX 115 kb)
705_2019_4211_MOESM2_ESM.jpg (380 kb)
Fig. S1 Pairwise comparisons between CQ1 and other strains based on the whole genome sequence (A) and an alignment of sequences of representative ENTV-2 strains based on the ORFx amino acid sequence (B). (JPEG 379 kb)
705_2019_4211_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 14 kb)


  1. 1.
    De las Heras M, Ortin A, Cousens C, Minguijon E, Sharp JM (2003) Enzootic nasal adenocarcinoma of sheep and goats. Curr Top Microbiol Immunol 275:201–223PubMedGoogle Scholar
  2. 2.
    Palmarini M, Fan H (2003) Molecular biology of jaagsiekte sheep retrovirus. Curr Top Microbiol Immunol 275:81–115PubMedGoogle Scholar
  3. 3.
    De las Heras M, García de Jalón JA, Minguijón E, Gray EW, Dewar P, Sharp JM (1995) Experimental transmission of enzootic intranasal tumors of goats. Vet Pathol 32:19–23CrossRefPubMedGoogle Scholar
  4. 4.
    Walsh SR, Linnerth-Petrik NM, Yu DL, Foster RA, Menzies PI, Diaz-Méndez A, Chalmers HJ, Wootton SK (2013) Experimental transmission of enzootic nasal adenocarcinoma in sheep. Vet Res 44:66CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Cousens C, Minguijon E, Dalziel RG, Ortin A, Garcia M, Park J, Gonzalez L, Sharp JM, De las Heras M (1999) Complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep. J Virol 73:3986–3993PubMedPubMedCentralGoogle Scholar
  6. 6.
    Ortin A, Cousens C, Minguijon E, Pascual Z, Villarreal MP, Sharp JM, Heras Mde L (2003) Characterization of enzootic nasal tumour virus of goats: complete sequence and tissue distribution. J Gen Virol 84:2245–2252CrossRefPubMedGoogle Scholar
  7. 7.
    Sid N, Belalmi NEH, Benhamza L, Ouhida S, Zebiri ME, Aydoğan A, Leroux C (2018) First case report of enzootic nasal adenocarcinoma in “Ouled Djellal” ewe in Algeria. Open Vet J 8:9–12CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Lin X, Hao XP, Zhao ZH, Yu WS, Gu YF, Bao GP (1995) Pathological study of goat nasal adenoma and adenocarcinoma. Acta Vet Zootechn Sin 5:456–461 (in Chinese) Google Scholar
  9. 9.
    Lei HY, Su JM, Ning LZ, Kang Y, Chen KY, Zeng DN (2006) Investigation and diagnosis of an enzootic nasal tumor of goat. Prog Vet Med 27:112–114 (in Chinese) Google Scholar
  10. 10.
    Liu F, Feng YC, Yan QG, Han GQ (2011) Diagnosis of an enzootic nasal tumor of goat in Sichuan. Anim Husban Vet Med 43:83–86 (in Chinese) Google Scholar
  11. 11.
    He Y, Zhang Q, Wang J, Zhou M, Fu M, Xu X (2017) Full-length genome sequence analysis of enzootic nasal tumor virus isolated from goats in China. Virol J 14:141CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Jiang JX, Lin YS, Jiang B, Mao KM, You W, Zhang JP, Hu QL (2017) Molecular epidemiology of enzootic nasal tumor virus on goats in Fujian. J FJ Agri Sci 32:837–841 (in Chinese) Google Scholar
  13. 13.
    Hou HY, Zhu DJ, Zhang DJ, Hu XM, Zhao RH, Dai Y (2018) Cloning and analysis of Gag gene of enzootic nasal tumor virus in goats. Chin Herb Sci 2:53–55 (in Chinese) Google Scholar
  14. 14.
    Xu G, Xian SM, Zeng ZY, Liang HY, Wang B, Huang T, Tang DY (2018) Diagnosis of a goat intranasal tumor. Chin Herb Sci 2:75–77 (in Chinese) Google Scholar
  15. 15.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Chao Ye
    • 1
  • Qingyuan Huang
    • 1
  • Tingting Chen
    • 1
  • Jiali Jiang
    • 1
  • Fengqing Hou
    • 1
  • Dongyi Xu
    • 1
  • Yuanyi Peng
    • 1
  • Rendong Fang
    • 1
    Email author
  • Jing Chen
    • 2
    Email author
  1. 1.College of Animal Science and TechnologySouthwest UniversityChongqingChina
  2. 2.Chongqing Academy of Animal SciencesChongqingChina

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