Advertisement

Archives of Virology

, Volume 164, Issue 5, pp 1459–1467 | Cite as

Establishment of a rescue system for porcine parvovirus using a seamless cloning method

  • Lingling Zhang
  • Dou Gao
  • Yongle Yu
  • Yebing Liu
  • Weiquan Liu
  • Jinxiang Li
  • Shangjin CuiEmail author
Brief Report
  • 89 Downloads

Abstract

In this study, we describe a novel and rapid method for the construction of a full-length infectious clone (pPPV). The constructed clone contained an engineered EcoRv site that served as a genetic marker and was shown to be infectious when transfected into a monolayer of PK-15 cells. The rescued virus (rPPV) of the infectious clone was found to be indistinguishable from wild-type virus BQ in terms of its biological properties. The generation of this PPV infectious clone provides a potentially powerful tool with which to elucidate the molecular pathogenesis of PPV.

Keywords

Porcine parvovirus Infectious clone Genetic marker Rescued virus Biological properties 

Notes

Acknowledgements

This work was supported by the Agricultural Science and Technology Innovation Program (ASTIP-IAS15), National Natural Science Foundation of China (NO. 31172349 & NO. 31172341) and the National Key Research and Development Program of China (No. 2016YFD0501003 & 2017YFD0502300).

Compliance with ethical standards

Conflicts of interest

All authors declare they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants and the animals studies was approved by the Science Research Department (in charge of animal welfare issue) of the Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (IAS-CAAS) (Beijing, China).

Informed consent

The author unanimously agreed to submit the manuscript to Archives of Virology.

References

  1. 1.
    Mayr A, Mahnel H (1964) Cultivation of hog cholera virus in pig kidney cultures with cytopathogenic effect. Zentralbl Bakteriol Orig 195:157–166Google Scholar
  2. 2.
    Cartwright SF, Huck RA (1967) Viruses isolated in association with herd infertility, abortions and stillbirths in pigs. Vet Rec 81:196–197Google Scholar
  3. 3.
    Joo HS, Donaldson-Wood CR, Johnson RH (1976) Observations on the pathogenesis of porcine parvovirus infection. Arch Virol 51:123–129CrossRefGoogle Scholar
  4. 4.
    Cui J, Wang X, Ren Y, Cui S, Li G, Ren X (2012) Genome sequence of Chinese porcine parvovirus strain PPV2010. J Virol 86:2379–2379CrossRefGoogle Scholar
  5. 5.
    Kim J, Chae C (2004) Concurrent presence of porcine circovirus type 2 and porcine parvovirus in retrospective cases of exudative epidermitis in pigs. Vet J 167:104–106CrossRefGoogle Scholar
  6. 6.
    Cotmore SF, Agbandje-McKenna M, Chiorini JA, Mukha DV, Pintel DJ, Qiu J, Söderlund-Venermo M, Tattersall P, Tijssen P, Gatherer D, Davison AJ (2014) The family Parvoviridae. Arch Virol 159:1239–1247CrossRefGoogle Scholar
  7. 7.
    Mengeling WL, Lager KM, Vorwald AC (2000) The effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance. Anim Reprod Sci 60–61:199–210CrossRefGoogle Scholar
  8. 8.
    Truyen U, Streck AF (2012) Porcine parvovirus. In: Zimmerman J, Karriker L, Ramirez A, Schwartz K, Stevenson G (eds) Diseases of swine 10. Wiley, Oxfort, pp 447–455Google Scholar
  9. 9.
    Cotmore SF, Sturzenbecker LJ, Tattersall P (1983) The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides. Virology 129:333–343CrossRefGoogle Scholar
  10. 10.
    Hao X, Lu Z, Sun P, Fu Y, Cao Y, Li P, Bai X, Bao H, Xie B, Chen Y, Li D, Liu Z (2011) Phylogenetic analysis of porcine parvoviruses from swine samples in China. Virol J 8:320CrossRefGoogle Scholar
  11. 11.
    Fernandes S, Boisvert M, Tijssen P (2011) Genetic elements in the VP region of porcine parvovirus are critical to replication efficiency in cell culture. J Virol 85:3025–3029CrossRefGoogle Scholar
  12. 12.
    Wang X, Ponnazhagan S, Srivastava A (1996) Rescue and replication of adeno-associated virus type 2 as well as vector DNA sequences from recombinant plasmids containing deletions in the viral inverted terminal repeats: selective encapsidation of viral genomes in progeny virions. J Virol 70:1668–1677Google Scholar
  13. 13.
    Pintel D, Dadachanji D, Astell CR, Ward DC (1983) The genome of minute virus of mice, an autonomous parvovirus, encodes two overlapping transcription units. Nucleic Acids Res 11:1019–1038CrossRefGoogle Scholar
  14. 14.
    Casal JI, Diazaroca E, Ranz AI, Manclus JJ (1990) Construction of an infectious genomic clone of porcine parvovirus: effect of the 5’-end on DNA replication. Virology 177:764–767CrossRefGoogle Scholar
  15. 15.
    Horiuchi M, Shinagawa M (1993) Construction of an infectious DNA clone of the Y 1 strain of canine parvovirus and characterization of the virus derived from the clone. Arch Virol 130:227–236CrossRefGoogle Scholar
  16. 16.
    Zhi N, Zadori Z, Brown KE, Tijssen P (2004) Construction and sequencing of an infectious clone of the human parvovirus B19. Virology 318:142–152CrossRefGoogle Scholar
  17. 17.
    Sun Y, Chen AY, Cheng F, Guan W, Johnson FB, Qiu J (2009) Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol 83:3956–3967CrossRefGoogle Scholar
  18. 18.
    Wang JY, Huang Y, Zhou MX, Hardwidge PR, Zhu GQ (2016) Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30. Virol J 13:104CrossRefGoogle Scholar
  19. 19.
    Zhang C, Song C, Chen C, Cui S, Miao L (2010) Reproductive failure in wild boars associated to porcine parvovirus infection and in vivo and in vitro characterization of the causal isolate. Trop Anim Health Prod 42:1611–1613CrossRefGoogle Scholar
  20. 20.
    Zhu B, Cai G, Hall EO, Freeman GJ (2007) In-FusionTM assembly: seamless engineering of multi-domain fusion proteins, modular vectors and mutations. BioTechniques 43:354–359CrossRefGoogle Scholar
  21. 21.
    Berns KI (1990) Parvoviridae and their replication. In: Fields BN, Knipe DM (eds) Virology, 2nd edn. Raven Press Ltd, New York, pp 1743–1760Google Scholar
  22. 22.
    Tuo D, Shen W, Yan P, Li X, Zhou P (2015) Rapid construction of stable infectious full-length cDNA clone of papaya leaf distortion mosaic virus using in-fusion cloning. Viruses 7:6241–6250CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Lingling Zhang
    • 1
    • 2
  • Dou Gao
    • 1
    • 2
  • Yongle Yu
    • 3
  • Yebing Liu
    • 4
  • Weiquan Liu
    • 3
  • Jinxiang Li
    • 5
  • Shangjin Cui
    • 1
    • 2
    Email author
  1. 1.Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijingChina
  2. 2.Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technology of Beijing, Ministry of AgricultureBeijingChina
  3. 3.State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological SciencesChina Agricultural UniversityBeijingChina
  4. 4.China Institute of Veterinary Drugs ControlBeijingChina
  5. 5.Chinese Academy of Agricultural SciencesBeijingChina

Personalised recommendations