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Archives of Virology

, Volume 164, Issue 5, pp 1445–1451 | Cite as

Analysis of genetic variation of porcine circovirus type 2 within pig populations in central China

  • Peng-Li Xu
  • Yu Zhao
  • Hui-Hua Zheng
  • Run-Bo Tian
  • Hao-Ying Han
  • Hong-Ying ChenEmail author
  • Lan-Lan ZhengEmail author
Brief Report
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Abstract

In order to investigate the genetic diversity of porcine circovirus type 2 (PCV2), 284 clinical tissue samples were collected from different pig farms in central China from 2015 to 2017. A total of 162 tissue samples (162/284, 57.04%) were positive for PCV2 by PCR, and subsequently, the complete genome of 36 of these PCV2 samples was cloned and sequenced. The sequencing results showed that 37 complete PCV2 sequences were obtained from 36 PCV2-positive clinical samples. These PCV2 strains were relatively conserved and extremely homologous to the representative classical PCV2 strains. Of these, 20 PCV2 strains belonged to genotype PCV2d, 14 belonged to PCV2b, and three others belonged to PCV2a. Coinfection with PCV2b and PCV2d was identified in one sample (DF-2). These results show that PCV2d may be gradually replacing PCV2b as the predominant PCV2 genotype in central China, and that other genotypes also exist in individual regions. The results of this study will aid in our understanding of the molecular epidemiology of PCV2.

Keywords

Porcine circovirus type 2 PCV2d Coinfection China 

Notes

Funding

This work was funded by the National Key Research and Development Programme (2018YFD0500800), the Science and Technology Innovation Team Plan of Henan (19IRTSTHN007), and the Science and Technology Opening Cooperation Project of Henan (182106000048).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhouPeople’s Republic of China
  2. 2.Zhengzhou Major Pig Disease Prevention and Control LaboratoryZhengzhouPeople’s Republic of China

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