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The first complete genomic sequence of cardamom mosaic virus, a member of the genus Macluravirus (family Potyviridae)

  • Sangeetha Elangovan
  • Nagesh Srikakulam
  • Gopal Pandi
  • Thomas Jacob
  • Usha Ramakrishnan
  • Venugopal Madhugiri Narayanarao
  • Jebasingh TennysonEmail author
Annotated Sequence Record
  • 67 Downloads

Abstract

The complete genome sequence of the KS isolate of cardamom mosaic virus (CdMV) was determined using transcriptome sequencing data from CdMV-infected Elettaria cardamomum as well as from overlapping cDNA clones made from RNA extracted from viral particles. The viral genome consists of 8249 nucleotides (nt) and encodes a large polyprotein of 2636 amino acids (aa). The polyprotein of CdMV shared 48.9%-67.4% aa sequence identity with other reported macluraviruses. Similar to the other members of genus Macluravirus, the genome of CdMV lacks the P1 coding region and the N-terminus of the HC-Pro coding region. The putative small open reading frame, PIPO, embedded within the P3 cistron, is preceded by a C(A)6 motif instead of G(A)6. Phylogenetic analysis based on the complete genome sequence aided the grouping of CdMV along with all other macluraviruses and showed that it is closely related to alpinia oxyphylla mosaic virus (AloMV). Among CdMV isolates, the KS isolate is most similar to the Appangala isolate based on disease symptoms and phylogeny.

Notes

Acknowledgements

This work was supported by the University Grants Commission (UGC), Government of India (File. No: 41-555/2012 (SR)).

Compliance with ethical standards

Conflict of interest

All authors declare no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

705_2019_4203_MOESM1_ESM.doc (35 kb)
Supplementary File 1: Primers used in the study (DOC 35 kb)
705_2019_4203_MOESM2_ESM.tif (401 kb)
Supplementary File 2: Figure depicting the read alignment coverage (TIFF 400 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant Sciences, School of Biological SciencesMadurai Kamaraj UniversityMaduraiIndia
  2. 2.Department of Plant Biotechnology, School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia
  3. 3.Department of Biomedical EngineeringOregon Health and Science UniversityPortlandUnited States
  4. 4.Indian Institute of Spices ResearchAppangalaIndia

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