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Archives of Virology

, Volume 164, Issue 5, pp 1433–1439 | Cite as

Innate immune response in astrocytes infected with herpes simplex virus 1

  • Yashvant D. Bansode
  • Debprasad Chattopadhyay
  • Bhaskar SahaEmail author
Brief Report

Abstract

Herpes simplex virus 1 (HSV-1), a double-stranded DNA virus, infects epithelial surfaces and establishes latency in the central nervous system, where astrocytes are a major immune cell type. Here, we report changes that occur in the expression of pathogen recognition receptors, such as Toll-like receptors, DNA and RNA sensors, interferons, and interferon-stimulated genes, when astrocytes are infected with HSV-1 strain F. We observed upregulation of Toll-like receptors 2, 6 and 9, MDA5, and DAI along with an increase in the expression of type I interferons and interferon-stimulated genes such as IFIT1, IFIT3 and RNase L. These genes encode proteins that mediate the antiviral immune response.

Abbreviations

AIM2

Absent in melanoma 2

cGAS

Cyclic GMP-AMP synthase

DAI

DNA-dependent activator of IFN-regulatory factors

DHX9

DExD/H-box helicase

eIF

Eukaryotic initiation factor

GAS

Gamma-interferon-activation sites

HSV-1

Herpes simplex virus 1

IFN

Interferon

IFN-R

Interferon receptor

IFI204

IFN-inducible gene 204

IFIT

Interferon-induced proteins with tetracopeptide repeats

IRES

Internal ribosome entry site

IRF

Interferon regulatory factor

ISG

Interferon-stimulated gene

ISRE

Interferon-stimulated response element

JAK

Janus kinase

MDA-5

Melanoma differentiation-associated protein 5

PAMP

Pathogen-associated molecular pattern

PKR

Protein kinase R

PRR

Pathogen recognition receptor

RIG-1

Retinoic-acid-inducible gene 1

RNaseL

Latent RNase

STAT

Signal transducer and activator of transcription

TLR

Toll-like receptor

Tyk-2

Tyrosine kinase 2

Notes

Acknowledgements

Y.D.B and B.S were supported by the Department of Biotechnology and Infect-eRA (DBT, BT/In /InfecteRA /33/BS/2016-17). A visit by B.S. to Case Western Reserve University was supported by P30 AI0362 19.

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interest.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Centre for Cell SciencePuneIndia
  2. 2.National Institute of Cholera and Enteric DiseasesKolkataIndia
  3. 3.Trident Academy of Creative TechnologyBhubaneswarIndia
  4. 4.National Institute of Traditional MedicineBelagaviIndia
  5. 5.Department of Molecular Biology and MicrobiologyCase Western Reserve UniversityClevelandUSA

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