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Archives of Virology

, Volume 164, Issue 5, pp 1419–1426 | Cite as

Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses

  • Humberto J. DebatEmail author
  • Nicolas BejermanEmail author
Brief Report

Abstract

Here, we report the identification and characterization of two novel viruses associated with bird’s-foot trefoil. Virus sequences related to those of enamoviruses (ssRNA (+); Luteoviridae; Enamovirus) and nucleorhabdoviruses (ssRNA (-); Rhabdoviridae; Nucleorhabdovirus) were detected in Lotus corniculatus transcriptome data. The genome of the tentatively named “bird’s-foot trefoil-associated virus 1” (BFTV-1) is a 13,626-nt-long negative-sense ssRNA. BFTV-1 encodes six predicted gene products in the antigenome orientation in the canonical order 3′-N-P-P3-M-G-L-5′. The genome of the proposed “bird’s-foot trefoil-associated virus 2” (BFTV-2) is 5,736 nt long with a typical 5΄-PO-P1-2-IGS-P3-P5-3′ enamovirus genome structure. Phylogenetic analysis indicated that BFTV-1 is closely related to datura yellow vein nucleorhabdovirus and that BFTV-2 clusters into a monophyletic lineage of legume-associated enamoviruses. This subclade of highly related and co-divergent legume-associated viruses provides insights into the evolutionary history of the enamoviruses.

Notes

Acknowledgements

We would like to express sincere gratitude to the generators of the underlying data used for this work: Dr. Ying Wang and Dr. Zhezhi Wang. By following open access practices and making accessible raw sequence data in public repositories available to the research community, they have promoted the generation of new knowledge and ideas.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sector.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Instituto de Patología Vegetal, Centro de Investigaciones AgropecuariasInstituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA)CórdobaArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

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