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Archives of Virology

, Volume 164, Issue 5, pp 1485–1488 | Cite as

Complete genome sequence of the virus isolate vB_BthM-Goe5 infecting Bacillus thuringiensis

  • Birthe Nordmann
  • Tobias Schilling
  • Michael Hoppert
  • Robert HertelEmail author
Annotated Sequence Record
  • 94 Downloads

Abstract

Bacillus thuringiensis (Bt) is non-pathogenic for humans and serves as a biological control agent in agriculture. Understanding its phages will help to prevent industrial production loss of Bt products and will lead to a better understanding of phages in general. The complete genome of the new B. thuringiensis phage isolate vB_BthM-Goe5 (Goe5) was sequenced, revealing a linear 157,804-bp-long dsDNA chromosome flanked by 2579-bp-long terminal repeats. It contains two tRNAs and 272 protein coding regions, 69 of which could be assigned with an annotation. Morphological investigation, using transmission electron microscopy, revealed Myoviridae morphology. The formation of a double baseplate upon tail sheath contraction indicates a link to the group of SPO1-related phages. Comparative genomics with all Bacillus-related viral genomes available in the NCBI genome database during this investigation indicated that Goe5 was a unique isolate, with Bacillus phage Bastille as its closest relative.

Notes

Acknowledgements

We would like to thank Professor Dr. Rolf Daniel for scientific advice and guidance, and Dr. Anja Poehlein for sequencing.

Funding

This project was funded by the Volkswagen Foundation (Re. 94045).

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Human and animal rights statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4187_MOESM1_ESM.docx (680 kb)
Supplementary material 1 (DOCX 680 kb)
705_2019_4187_MOESM2_ESM.xlsx (65 kb)
Supplementary material 2 (XLSX 65 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and GeneticsGeorg-August-University GöttingenGöttingenGermany
  2. 2.Department of General Microbiology, Institute of Microbiology and GeneticsGeorg-August-University GöttingenGöttingenGermany

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