Archives of Virology

, Volume 164, Issue 5, pp 1485–1488 | Cite as

Complete genome sequence of the virus isolate vB_BthM-Goe5 infecting Bacillus thuringiensis

  • Birthe Nordmann
  • Tobias Schilling
  • Michael Hoppert
  • Robert HertelEmail author
Annotated Sequence Record


Bacillus thuringiensis (Bt) is non-pathogenic for humans and serves as a biological control agent in agriculture. Understanding its phages will help to prevent industrial production loss of Bt products and will lead to a better understanding of phages in general. The complete genome of the new B. thuringiensis phage isolate vB_BthM-Goe5 (Goe5) was sequenced, revealing a linear 157,804-bp-long dsDNA chromosome flanked by 2579-bp-long terminal repeats. It contains two tRNAs and 272 protein coding regions, 69 of which could be assigned with an annotation. Morphological investigation, using transmission electron microscopy, revealed Myoviridae morphology. The formation of a double baseplate upon tail sheath contraction indicates a link to the group of SPO1-related phages. Comparative genomics with all Bacillus-related viral genomes available in the NCBI genome database during this investigation indicated that Goe5 was a unique isolate, with Bacillus phage Bastille as its closest relative.



We would like to thank Professor Dr. Rolf Daniel for scientific advice and guidance, and Dr. Anja Poehlein for sequencing.


This project was funded by the Volkswagen Foundation (Re. 94045).

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

Human and animal rights statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

705_2019_4187_MOESM1_ESM.docx (680 kb)
Supplementary material 1 (DOCX 680 kb)
705_2019_4187_MOESM2_ESM.xlsx (65 kb)
Supplementary material 2 (XLSX 65 kb)


  1. 1.
    Logan NA, De Vos P (2009) Systematic bacteriology. Springer, New YorkGoogle Scholar
  2. 2.
    Hedges SB (2002) The origin and evolution of model organisms. Nat Rev Genet 3:838–849. CrossRefGoogle Scholar
  3. 3.
    Calendar R (2006) The bacteriophages. Oxford University Press, OxfordGoogle Scholar
  4. 4.
    Klumpp J, Lavigne R, Loessner MJ, Ackermann HW (2010) The SPO1-related bacteriophages. Arch Virol 155:1547–1561. CrossRefGoogle Scholar
  5. 5.
    Hendriksen NB, Hansen BM (2006) Detection of Bacillus thuringiensis kurstaki HD1 on cabbage for human consumption. FEMS Microbiol Lett 257:106–111. CrossRefGoogle Scholar
  6. 6.
    Day M, Ibrahim M, Dyer D, Bulla L (2014) Genome Sequence of Bacillus thuringiensis subsp. kurstaki Strain HD-1. Genome Announc.
  7. 7.
    Parker ML, Eiserling FA (1983) Bacteriophage SPO1 structure and morphogenesis. I. Tail structure and length regulation. J Virol 46:239–249. Google Scholar
  8. 8.
    Willms IM, Hoppert M, Hertel R (2017) Characterization of Bacillus subtilis Viruses vB_BsuM-Goe2 and vB_BsuM-Goe3. Viruses 9:146. CrossRefGoogle Scholar
  9. 9.
    Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. CrossRefGoogle Scholar
  10. 10.
    Seemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069. CrossRefGoogle Scholar
  11. 11.
    Jones P, Binns D, Chang H-Y et al (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30:1236–1240. CrossRefGoogle Scholar
  12. 12.
    Altschul SF, Gish W, Miller W et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410. CrossRefGoogle Scholar
  13. 13.
    Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci 106:19126–19131. CrossRefGoogle Scholar
  14. 14.
    Kurtz S, Phillippy A, Delcher AL et al (2004) Versatile and open software for comparing large genomes. Genome Biol 5:R12. CrossRefGoogle Scholar
  15. 15.
    Sauder AB, Quinn MR, Brouillette A et al (2016) Genomic characterization and comparison of seven Myoviridae bacteriophage infecting Bacillus thuringiensis. Virology 489:243–251. CrossRefGoogle Scholar
  16. 16.
    Gillis A, Mahillon J (2014) Phages preying on Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis: past, present and future. Viruses 6:2623–2672. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and GeneticsGeorg-August-University GöttingenGöttingenGermany
  2. 2.Department of General Microbiology, Institute of Microbiology and GeneticsGeorg-August-University GöttingenGöttingenGermany

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