Archives of Virology

, Volume 164, Issue 5, pp 1469–1473 | Cite as

Genomic characterization of a novel recombinant porcine astrovirus isolated in northeastern China

  • Chengxue Zhao
  • Chen Chen
  • Ying Li
  • Shuren Dong
  • Kun Tan
  • Yuhui Tian
  • Lilin Zhang
  • Jinhai Huang
  • Lei ZhangEmail author
Annotated Sequence Record


Porcine astroviruses (PAstVs), are widely distributed viruses that are highly prevalent in swine herds. In this study, a novel type 4 porcine astrovirus strain (designated as PAstV4/Tianjin/2018) was identified in a fecal sample from a diarrheal piglet in Tianjin, China and its complete genomic sequence was determined by RT-PCR. Sequence analysis showed that this strain had a capsid protein with a highly variable C-terminal domain, a typical ribosomal frameshifting signal, and a conserved subgenomic promoter sequence. Recombination analysis indicated that PAstV4/Tianjin/2018 was a novel recombinant strain, and a recombination breakpoint was identified at nt position 4220 of the genome. The novel recombinant porcine astrovirus identified in China will be useful for understanding the origin, genetic diversity, and evolution of enteric viruses.



This work was supported by the National Key Research Project of China (2016YFD050030), the underprop project of Tianjin Science and Technology Committee in China (16YFZCNC00640 and 17JCZDJC33900), and College Students’ Innovation and Entrepreneurship Training Project (201810056103).

Compliance with ethical standards

Animal and human rights statement

Animal experiments were performed in compliance with the regulations set by Tianjin University Institutional Animal Care and Use Committee and were approved by the veterinarian authorities of Tianjin Animal Inspection Institute. This study was carried out in strict accordance with the guidelines in the Guide for the Care and Use of Laboratory Animals in China.

Supplementary material

705_2019_4162_MOESM1_ESM.doc (550 kb)
Supplementary material 1 (DOC 550 kb)


  1. 1.
    Monroe SS, Jiang B, Stine SE, Koopmans M, Glass RI (1993) Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol 67:3611–3614Google Scholar
  2. 2.
    Bridger JC (1980) Detection by electron microscopy of caliciviruses, astroviruses and rotavirus-like particles in the faeces of piglets with diarrhoea. Vet Rec 107:532-aGoogle Scholar
  3. 3.
    Bosch A, Pinto RM, Guix S (2014) Human astroviruses. Clin Microbiol Rev 27:1048–1074. CrossRefGoogle Scholar
  4. 4.
    Brnić D, Jemeršić L, Keros T, Prpić J (2014) High prevalence and genetic heterogeneity of porcine astroviruses in domestic pigs. Vet J 202:390–392CrossRefGoogle Scholar
  5. 5.
    De Benedictis P, Schultz-Cherry S, Burnham A, Cattoli G (2011) Astrovirus infections in humans and animals—molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol 11:1529–1544. CrossRefGoogle Scholar
  6. 6.
    Pankovics P, Boros Á, Kiss T, Delwart E, Reuter G (2015) Detection of a mammalian-like astrovirus in bird, European roller (Coracias garrulus). Infect Genet Evol 34:114–121CrossRefGoogle Scholar
  7. 7.
    Xiao CT, Giménez-Lirola LG, Gerber PF, Jiang YH, Halbur PG, Opriessnig T (2013) Identification and characterization of novel porcine astroviruses (PAstVs) with high prevalence and frequent co-infection of individual pigs with multiple PAstV types. J Gen Virol 94:570CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Life SciencesTianjin UniversityTianjinChina
  2. 2.Tianjin Center of Animal Disease Preventive and ControlTianjinChina
  3. 3.Ninghe Breeding Pig Farm of TianjinTianjinChina

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