Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China
A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus “Trichoderma harzianum bipartite mycovirus 1” (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.
At present, only three mycoviruses infecting Trichoderma have been described [1, 2, 3]. In 2009, Jom-in and Akarapisan provided the first description of two mycoviruses, with sizes of 0.7 kb and 1.1 kb, respectively, isolated from Trichoderma . Later, Yun et al. suggested that mycoviruses from Lentinula edodes were widespread in Korea and isolated 32 different dsRNA-containing viruses from 315 strains of Trichoderma spp. . More recently, Lee et al. isolated an unclassified mycovirus from Trichoderma atroviride, naming it “Trichoderma atroviride mycovirus 1” (TaMV 1) . To expand the list of mycoviruses infecting Trichoderma spp., we have isolated 152 Trichoderma spp. from soil samples obtained from the Chinese provinces of Xinjiang, Inner Mongolia, Heilongjiang, and Jilin. These isolates were classified at the species level based on morphological properties and molecular data (sequencing of the ITS region, the translation elongation factor 1-α (tef1-α) gene, and the RNA polymerase subunit II (rpb2) gene). In an effort to identify new Trichoderma viruses, all of these isolates were screened for the presence of mycoviruses (Supplemental Table 1).
Identification of a novel Trichoderma mycovirus
The larger segment of 2088 bp was named dsRNA1, and the smaller segment of 1634 bp was named dsRNA2. Both included a poly(A) tail. The G+C content of the dsRNA1 segment was 50.0%, and for dsRNA2 it was 50.3%. Possible ORFs were found using the NCBI ORF finder tool (https://www.ncbi.nlm.nih.gov/orffinder/). Each dsRNAs contained a single ORF in the positive-sense strand. The 5′ UTR of dsRNA1 was 78 bp long, and the 3′ UTR was 114 bp long; they had no hairpin structures in the UTR regions. The 5′ UTR of dsRNA2 was 105 bp long, while the 3′ UTR was 584 bp long; the structure of UTR regions was the same as for dsRNA1. The 5′ UTRs of dsRNA1 and dsRNA2 had the same conserved element (CUGAGUUAACAAGCCACUGUUUUACUCUCGU), which is necessary for virus replication. A BLASTX search for homologues of ORF1 (nt 79-1974) showed that ORF1 most likely encodes an RNA-dependent RNA polymerase (RdRP) of 631 amino acids with a molecular weight of 72.3 kDa. The C-terminal domain and catalytic palm subdomain were detected in the regions encompassing amino acids 300-448 and 326-454, respectively, using InterPro (http://www.ebi.ac.uk/interpro). Likewise, ORF2 (nt 106-1050) was predicted to encode a hypothetical protein of 314 amino acids with a molecular weight of 37.5 kDa with similarity to other proteins of unknown function or coat proteins from unclassified dsRNA mycoviruses and members of the family Partitiviridae (Supplementary Table 3).
We thank Irina S. Druzhinina (Institute of Chemical, Environmental and Bioscience Engineering, Department of Biochemical Technology, Microbiology and Applied Genomic Group, TU WIEN, Gumpendorferstrasse 1a, A1060, Vienna, Austria) for her insights and suggestions for the identification of the Trichoderma strains.
This work was supported by the National Key Research and Development Plan (Chemical fertilizer and pesticide reducing efficiency synergistic technology research and development): Research and demonstration of a new high efficiency biocide (2017YFD0201100-2017YFD0201102) granted to Xiliang Jiang; Research and demonstration of technology integration on reducing chemical fertilizer and pesticide application in open field vegetables (2018YFD0201200-2018YFD0201202) granted to Mei Li; Demonstration of comprehensive prevention and control technology of non-point source pollution in main vegetable producing areas of Huang Huai Hai (SQ2018YFD080026) granted to Beilei Wu; Work in València was supported by Spain’s Agencia Estatal de Investigación - FEDER grant BFU2015-65037-P granted to Santiago F. Elena.
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Conflict of interest
None of the authors have conflicts of interest.
This article does not contain any studies with human participants performed by any of the authors.
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