Advertisement

Archives of Virology

, Volume 164, Issue 2, pp 653–656 | Cite as

Asian black bear (Ursus thibetanus) picornavirus related to seal aquamavirus A

  • Haoning Wang
  • Wen ZhangEmail author
  • Shixiing Yang
  • Ning Kong
  • Hai Yu
  • Hao Zheng
  • Fei Gao
  • Wu Tong
  • Liwei Li
  • Xiaolong Wang
  • Xutao Deng
  • Eric Delwart
  • Tongling ShanEmail author
Annotated Sequence Record
  • 89 Downloads

Abstract

The complete genome of a bear picornavirus 1 (BePV-1) in the viscera of an Asian black bear (Ursus thibetanus) from China was characterized using viral metagenomics and RT-PCR/Sanger sequencing. The genome of BePV1 is 6703 nt long, contains a type-IV IRES 5’UTR with the ‘8-like’ motif, encodes a 2053-aa-long polyprotein showing a 3-4-4 organization pattern and two 2A genes. BePV-1 showed the highest overall genome nucleotide sequence identity of 71.7% to a picornavirus genome from an Arctic ringed seal (Phoca hispida) from Canada, classified as a member of the species Aquamavirus A, currently the only one in the genus Aquamavirus. Phylogenetic and genetic distance analyses of P1 and 3D indicated that Asian bear picornavirus (aquamavirus B) represents the second sequenced member of the genus Aquamavirus.

Notes

Acknowledgements

This work was partly supported by the National Key Research and Development Programs of China (no. 2017YFC1200201 and no. 2016YFD0500103) and the National Natural Science Foundation (no. 31872478 and 81741062).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.

Supplementary material

705_2018_4101_MOESM1_ESM.docx (14 kb)
Supplementary material 1 (DOCX 13 kb)

References

  1. 1.
    Asnani M, Kumar P, Hellen CU (2015) Widespread distribution and structural diversity of type IV IRESs in members of Picornaviridae. Virology 478:61–74CrossRefGoogle Scholar
  2. 2.
    Boros A, Pankovics P, Simmonds P, Kiss T, Phan TG, Delwart E, Reuter G (2018) Genomic analysis of a novel picornavirus from a migratory waterfowl, greater white-fronted goose (Anser albifrons). Arch Virol 163:1087–1090CrossRefGoogle Scholar
  3. 3.
    Kapoor A, Victoria J, Simmonds P, Wang C, Shafer RW, Nims R, Nielsen O, Delwart E (2008) A highly divergent picornavirus in a marine mammal. J Virol 82:311–320CrossRefGoogle Scholar
  4. 4.
    King AMQ, Lefkowitz EJ, Mushegian AR, Adams MJ, Dutilh BE, Gorbalenya AE, Harrach B, Harrison RL, Junglen S, Knowles NJ, Kropinski AM, Krupovic M, Kuhn JH, Nibert ML, Rubino L, Sabanadzovic S, Sanfacon H, Siddell SG, Simmonds P, Varsani A, Zerbini FM, Davison AJ (2018) Changes to taxonomy and the international code of virus classification and nomenclature ratified by the International Committee on Taxonomy of Viruses (2018). Arch Virol 163:2601–2631CrossRefGoogle Scholar
  5. 5.
    Reuter G, Boros A, Kiss T, Delwart E, Pankovics P (2014) Complete genome characterization of mosavirus (family Picornaviridae) identified in droppings of a European roller (Coracias garrulus) in Hungary. Arch Virol 159:2723–2729CrossRefGoogle Scholar
  6. 6.
    Shan T, Li L, Simmonds P, Wang C, Moeser A, Delwart E (2011) The fecal virome of pigs on a high-density farm. J Virol 85:11697–11708CrossRefGoogle Scholar
  7. 7.
    Zell R, Delwart E, Gorbalenya AE, Hovi T, King AMQ, Knowles NJ, Lindberg AM, Pallansch MA, Palmenberg AC, Reuter G, Simmonds P, Skern T, Stanway G, Yamashita T, Ictv Report C (2017) ICTV virus taxonomy profile: Picornaviridae. J Gen Virol 98:2421–2422CrossRefGoogle Scholar
  8. 8.
    Zell R (2018) Picornaviridae-the ever-growing virus family. Arch Virol 163:299–317CrossRefGoogle Scholar
  9. 9.
    Zhang W, Yang S, Shan T, Hou R, Liu Z, Li W, Guo L, Wang Y, Chen P, Wang X, Feng F, Wang H, Chen C, Shen Q, Zhou C, Hua X, Cui L, Deng X, Zhang Z, Qi D, Delwart E (2017) Virome comparisons in wild-diseased and healthy captive giant pandas. Microbiome 5:90CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Haoning Wang
    • 1
    • 2
  • Wen Zhang
    • 3
    Email author
  • Shixiing Yang
    • 3
  • Ning Kong
    • 2
  • Hai Yu
    • 2
  • Hao Zheng
    • 2
  • Fei Gao
    • 2
  • Wu Tong
    • 2
  • Liwei Li
    • 2
  • Xiaolong Wang
    • 1
  • Xutao Deng
    • 4
  • Eric Delwart
    • 4
  • Tongling Shan
    • 2
    • 5
    Email author
  1. 1.College of Wildlife ResourceNortheast Forestry UniversityHarbinChina
  2. 2.Department of Swine Infectious Disease, Shanghai Veterinary Research InstituteChinese Academy of Agricultural SciencesShanghaiChina
  3. 3.School of MedicineJiangsu UniversityZhenjiangChina
  4. 4.Blood Systems Research InstituteSan FranciscoUSA
  5. 5.Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhouChina

Personalised recommendations