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Archives of Virology

, Volume 164, Issue 2, pp 617–620 | Cite as

Sequence analysis and genomic organization of a novel chuvirus, Tàiyuán leafhopper virus

  • Hui Wang
  • Yan LiuEmail author
  • Wenwen Liu
  • Mengji Cao
  • Xifeng Wang
Annotated Sequence Record

Abstract

A novel unsegmented chuvirus was found in a leafhopper (Psammotettix alienus) and tentatively named “Tàiyuán leafhopper virus” (TYLeV). The full genome of TYLeV is 14,670 nucleotides in length and includes four ORFs encoding glycoprotein, nucleocapsid protein, RNA-dependent RNA polymerase and an uncharacterized protein, respectively. These genomic features are similar to those of the chuvirus Scaldis River bee virus, but the virus is highly divergent from known chuviruses and other members in the order Mononegavirales, with amino acid sequence identities of only 13.6–33% for the RNA-dependent RNA polymerase (RdRp). On the basis of genomic features, phylogenetic analysis and comparisons of RdRp sequences, we suggest that this virus is a member of a new species of chuvirus in the proposed genus Mivirus.

Notes

Funding

This research was supported by the Inter-Governmental S&T Cooperation Proposal (2016YFE0131000) and the National Key Research and Development Program of China (2017YFD0201700).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

No experimental work with humans was done in this study.

Supplementary material

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Fig. S1 Amino acid sequence alignment of core RdRp motifs of TYLeV, chuviruses, and selected viruses of the order Mononegavirales (JPEG 341 kb)
705_2018_4075_MOESM2_ESM.docx (14 kb)
Supplementary material 2 (DOCX 14 kb)
705_2018_4075_MOESM3_ESM.docx (19 kb)
Supplementary material 3 (DOCX 19 kb)
705_2018_4075_MOESM4_ESM.fa (14 kb)
Supplementary material 4 (fa 14 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  2. 2.National Citrus Engineering Research Center, Citrus Research InstituteSouthwest UniversityChongqingChina

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