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The complete genome sequence analysis of West Nile virus strains isolated in Slovakia (central Europe)

  • Petra Drzewnioková
  • Luisa Barzon
  • Elisa Franchin
  • Enrico Lavezzo
  • Tamás Bakonyi
  • Juraj Pistl
  • Tomáš Csank
Brief Report

Abstract

The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3, E159 and E231) and in non-structural (NS1109, NS5259, NS5310 and NS5600) proteins. Observed amino acid substitutions Phe3 and Ser231 were common only within the lineage VII Koutango strain isolated from Rhipicephalus guilhoni tick in Senegal. Further research could reveal whether these substitutions influence the biological properties of WNV, including virulence and neuroinvasiveness.

Notes

Acknowledgements

The authors thank to Associate Professor, DVM. Dagmar Heinová, PhD. for critical review of the manuscript and to Mrs. Vladimíra Antožiová for technical assistance.

Compliance with ethical standards

Funding

This study was supported by grants VEGA 1/0729/16 and NKFIH K 120118.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (PDF 211 kb)
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Supplementary material 5 (PDF 151 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Microbiology and ImmunologyUniversity of Veterinary Medicine and Pharmacy in KošiceKošiceSlovakia
  2. 2.Department of Molecular MedicineUniversity of PadovaPadovaItaly
  3. 3.Microbiology and Virology UnitPadova University HospitalPadovaItaly
  4. 4.Institute of VirologyUniversity of Veterinary Medicine ViennaViennaAustria
  5. 5.Department of Microbiology and Infectious DiseasesUniversity of Veterinary MedicineBudapestHungary

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