The complete genome sequence analysis of West Nile virus strains isolated in Slovakia (central Europe)
The present study reports the first complete genome sequence analysis of West Nile virus (WNV) strains isolated from brain samples from raptors. The results prove the circulation of closely related WNV lineage II strains in central Europe and genetic analysis revealed seven amino acid substitutions in structural (PrM3, E159 and E231) and in non-structural (NS1109, NS5259, NS5310 and NS5600) proteins. Observed amino acid substitutions Phe3 and Ser231 were common only within the lineage VII Koutango strain isolated from Rhipicephalus guilhoni tick in Senegal. Further research could reveal whether these substitutions influence the biological properties of WNV, including virulence and neuroinvasiveness.
The authors thank to Associate Professor, DVM. Dagmar Heinová, PhD. for critical review of the manuscript and to Mrs. Vladimíra Antožiová for technical assistance.
Compliance with ethical standards
This study was supported by grants VEGA 1/0729/16 and NKFIH K 120118.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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