Vector competence analysis of two Aedes aegypti lineages from Bello, Colombia, reveals that they are affected similarly by dengue-2 virus infection
Dengue is the second most prevalent vector-borne disease after malaria in Colombia. It is caused by dengue virus, an arbovirus that exhibits high epidemic power, which is evidenced by its occurrence in more than 80% of the country, largely because of the extensive dispersion of the mosquito vector Aedes aegypti. The existence of two lineages of Ae. aegypti has been proposed based on genetic differences at the mitochondrial level, and they have been reported to circulate in similar proportions in the municipality of Bello (Colombia). It has been suggested that the differentiation of these lineages could influence features such as vector competence (VC) and life table. With the aim of testing this hypothesis, female mosquitoes from both lineages collected from Bello were orally challenged with dengue virus serotype 2 (strain D2-HAN) to measure infection, dissemination, survival and fecundity. Analysis of VC showed an increase in viral titer over time; however, no significant differences were observed between the lineages. The survival rate was not different between the infected lineages, but comparing lineages, it was lower in infected mosquitoes, which may affect the intensity of transmission. Finally, we conclude that the genetic differentiation of Ae. aegypti into lineages did not confer differences in epidemiological status when the mosquitoes were infected with this D2 serotype strain.
We thank A. Trujillo-Correa at Grupo PECET-UdeA for methodological guidelines, Jeiczon Jaimes-Dueñez at Grupo BCEI for guidelines in population genetics analysis, and FJ Díaz at Grupo Inmunovirología-UdeA for virus stock.
Compliance with ethical standards
This study was funded by Departamento Administrativo de Ciencia, Tecnología e Innovación-COLCIENCIAS, Colombia (grant number 111572553478) and project Sostenibilidad UdeA 2016.
Conflict of interest
The authors declare no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors. For animals, all applicable international, national, and institutional guidelines for the care and use of animals were followed. The use of animals was approved by CEEA (Comité de Ética para la Experimentación con Animales) of UdeA.
- 2.Instituto Nacional de Salud (2014) Ministerio de Salud y Proteccion Social: Protocolo de vigilancia en Salud PúblicaGoogle Scholar
- 14.Jaimes-Dueñez J, Arboleda S, Triana-Chávez O, Gómez-Palacio A (2015) Spatio-temporal distribution of Aedes aegypti (Diptera : Culicidae) mitochondrial lineages in cities with distinct Dengue incidence rates suggests complex population dynamics of the Dengue vector in Colombia. PLoS Negl Trop Dis 9:1–21CrossRefGoogle Scholar
- 16.DANE (2016) Demografia y Poblacion. https://www.dane.gov.co/index.php/estadisticas-por-tema/demografia-y-poblacion/proyecciones-de-poblacion. Accessed 4 Oct 2018
- 17.González CR, Jercic MI, Reyes C et al (2008) A pictorial key to the genera of Culicidae (Diptera) from Chile of medical importance. Acta Entomol Chil 32:35–42Google Scholar
- 23.Halls TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- 25.Rambaut A. (2006–2014) FigTree Tree Figure Drawing Tool, Version 1.4.2. http://tree.bio.ed.ac.uk/software/figtree/. Accessed 4 Oct 2018
- 26.Steinly B, Novak RJ, Webb DW (1991) A new method for monitoring mosquito oviposition in artificial and natural containers. J Am Mosq Control Assoc 7:649–650Google Scholar
- 29.Camacho DE, Guzmán MG, Morier L et al (1999) Estudio de algunas propiedades biológicas de 3 cepas de dengue 2 con diferencias en sus secuencias nucleotídicas. Rev Cuba Med Trop 51:177–180Google Scholar
- 30.Instituto de Medicina Tropical Pedro Kourí (2013) Técnicas de laboratorio para el diagnóstico y la caracterización de los virus del dengue. Laboratorio de Arbovirus, Departamento de Virología La Habana, Cuba. Rev Inst Med Trop, pp 1–133Google Scholar
- 31.Galun R (1967) Feeding stimuli and artificial feeding. Bull World Health Organ 36:590–593Google Scholar
- 32.Cosgrove JB, Wood RJ, Petrić D et al (1994) A convenient mosquito membrane feeding system. J Am Mosq Control Assoc 10:434–436Google Scholar
- 33.Hagen H, Grunewald J (1990) Routine Blood-feding of Aedes aegypti via a new membrane. Oper Sci Notes 6:535–536Google Scholar
- 34.Foggie T, Achee N (2009) Standard operating procedures: rearing Aedes aegypti for the HITSS and Box Laboratory Assays Training Manual v1.0, pp 1–18Google Scholar
- 36.Turell M, Rossignol P, Rossi C, Bailey C (1984) Enhanced arboviral transmission by mosquitoes that concurrently ingested microfilariae. Science 80:225Google Scholar
- 38.GraphPad Prism 6.01 Software (2012). https://www.graphpad.com/www/data-analysis-resource-center/. Accessed 4 Oct 2018
- 47.Bosio CF, Fulton RE, Salasek ML et al (2000) Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti. Genetics 156:687–698Google Scholar
- 49.Carrington LB, Simmons CP (2014) Human to mosquito transmission of dengue viruses. Front Immunol 5(June):1–8Google Scholar
- 50.Mousson L, Vazeille M, Chawprom S, Prajakwong S, Rodhain F (2002) Genetic structure of Aedes aegypti populations in Chiang Mai (Thailand) and relation with dengue transmission. Science 7:865–872Google Scholar
- 53.Rosen L, Rosemboom LE, Gubler D, Lien JC, Chaniotis BN (1985) Comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and Japanese encephalitis viruses. ASTMH 34:603–615Google Scholar
- 55.Aliota MT, Walker E, Yepes A, Velez ID, Christensen BM, Osorio JE (2016) The wMel strain of wolbachia reduces transmission of chikungunya virus in Aedes aegypti. PLoS Negl Trop Dis 2016:13Google Scholar