Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells
- 214 Downloads
Bovine viral diarrhea virus (BVDV) is an important pathogen in cattle that causes economic losses in livestock industries. Autophagy is an essential cell system for the maintenance of homeostasis and is induced by various triggers, including infection by viruses. BVDV infection leads to autophagy in order to enhance its replication in cells. In this study, we investigated the effect of BVDV non-structural proteins on the induction of autophagosomes. We found that NS4B alone could induce autophagosomes, suggesting a novel and important function of NS4B in BVDV replication.
We thank Dr. Atsushi Miyawaki (Riken, Japan) for the Venus/pCS2 plasmid.
Compliance with ethical standards
Conflict of interest
There is no conflict of interest associated with this article.
Research involving human participants and/or animals
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent is not required because no human participants were involved in this article.
- 3.Behrens SE, Grassmann CW, Thiel HJ, Meyers G, Tautz N (1998) Characterization of an autonomous subgenomic pestivirus RNA replicon. J Virol 72:2364–2372Google Scholar
- 6.Warrener P, Collett MS (1995) Pestivirus NS3 (p80) protein possesses RNA helicase activity. J Virol 69:1720–1726Google Scholar
- 15.Lai VC, Kao CC, Ferrari E, Park J, Uss AS, Wright-Minogue J, Hong Z, Lau JY (1999) Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase. J Virol 73:10129–10136Google Scholar
- 16.Zhong W, Gutshall LL, Del Vecchio AM (1998) Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus. J Virol 72:9365–9369Google Scholar
- 21.Liang Q, Luo Z, Zeng J, Chen W, Foo SS, Lee SA, Ge J, Wang S, Goldman SA, Zlokovic BV, Zhao Z, Jung JU (2016) Zika virus NS4A and NS4B proteins deregulate Akt-mTOR signaling in human fetal neural stem cells to inhibit neurogenesis and induce autophagy. Cell Stem Cell 19:663–671CrossRefGoogle Scholar